Proteomics

Dataset Information

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Na2CO3-responsive proteins in chloroplasts from Puccinellia tenuiflora leaves


ABSTRACT: As the organelles in which photosynthesis occurs, chloroplasts are extremely susceptible to saline-alkali stress. Proteins from chloroplast were identified and quantified by isobaric tags for relative and absolute quantification (iTRAQ) strategy, and searched with both of Uniprot Liliposida database and NCBInr database. In each of the labeling experiments, approximately 700 proteins were identified, and 121 abundance changed proteins were identified in chloroplast under the condition of Na2CO3 treatment. While according to the subcellular localization prediction, 102 chloroplast localization abundance changed proteins were used for further analysis of the responsive mechanism of the chloroplast under the conditions of Na2CO3 treatment. The 102 proteins were classified into nine functional categories, including photosynthesis, energy metabolism, other metabolisms, stress and defense, membrane and transporting, signaling, chloroplast structure, gene expression, protein synthesis and folding, and miscellaneous. The abundance patterns of these proteins highlight the Na2CO3-responsive chloroplast structure modulation and a series of photosynthetic regulatory mechanisms, such as thermal dissipation, state transition, cyclic electron transport, photorespiration, repair of photodamaged PSII, alteration of PSI activity, and ROS homeostasis.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Oryza Sativa (rice)

TISSUE(S): Plant Cell, Shoot

SUBMITTER: Shaojun Dai  

LAB HEAD: Shaojun Dai

PROVIDER: PXD005491 | Pride | 2021-09-08

REPOSITORIES: Pride

Dataset's files

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20120309_ZhangHeng_A_F1.wiff Wiff
20120309_ZhangHeng_A_F1.wiff.mtd Wiff
20120309_ZhangHeng_A_F1.wiff.scan Wiff
20120309_ZhangHeng_A_F10.wiff Wiff
20120309_ZhangHeng_A_F10.wiff.mtd Wiff
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Publications

Na<sub>2</sub>CO<sub>3</sub>-responsive Photosynthetic and ROS Scavenging Mechanisms in Chloroplasts of Alkaligrass Revealed by Phosphoproteomics.

Suo Jinwei J   Zhang Heng H   Zhao Qi Q   Zhang Nan N   Zhang Yongxue Y   Li Ying Y   Song Baohua B   Yu Juanjuan J   Cao Jianguo J   Wang Tai T   Luo Ji J   Guo Lihai L   Ma Jun J   Zhang Xumin X   She Yimin Y   Peng Lianwei L   Ma Weimin W   Guo Siyi S   Miao Yuchen Y   Chen Sixue S   Qin Zhi Z   Dai Shaojun S  

Genomics, proteomics & bioinformatics 20200601 3


Alkali-salinity exerts severe osmotic, ionic, and high-pH stresses to plants. To understand the alkali-salinity responsive mechanisms underlying photosynthetic modulation and reactive oxygen species (ROS) homeostasis, physiological and diverse quantitative proteomics analyses of alkaligrass (Puccinellia tenuiflora) under Na<sub>2</sub>CO<sub>3</sub> stress were conducted. In addition, Western blot, real-time PCR, and transgenic techniques were applied to validate the proteomic results and test t  ...[more]

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