Proteomics

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Na2CO3-responsive phosphoproteins in Puccinellia tenuiflora leaves


ABSTRACT: Reversible protein phosphorylation also play crucial roles in plant salt resistance. In our syudy, 62 Na2CO3-responsive phosphoproteins were found in leaves by isobaric tags for relative and absolute quantification (iTRAQ) labeling. These Na2CO3-responsive phosphoproteins were sorted into seven functional categories. Among them, most of photosynthetic proteins including 11 light harvesting proteins, five PS II protein, and ten Calvin cycle-related protein (i.e. RuBisCO activase, RuBisCO large subunit, glyceraldehyde-3-phosphate dehydrogenase, and Phosphoglycerate kinase), three ATP synthase subunits, and other carbohydrate and energy metabolism related proteins were identified increased in phosphorylation level. While two starch and sucrose metabolism related protein (e.g. UDP-glucose 6-dehydrogenase), one stress response protein, one membrane and transporting protein, and most of gene expression, protein synthesis and turnover related proteins were identified inhibited phosphorylation under Na2CO3 treatment. In addition, calmodulin, inactive receptor kinase, VWA copine domain containing protein, and tetraspanin family protein participate in signaling pathways were identified enhanced phosphorylation under Na2CO3.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Oryza Sativa (rice)

TISSUE(S): Shoot

SUBMITTER: Shaojun Dai  

LAB HEAD: Shaojun Dai

PROVIDER: PXD005471 | Pride | 2021-08-30

REPOSITORIES: Pride

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Publications

Na<sub>2</sub>CO<sub>3</sub>-responsive Photosynthetic and ROS Scavenging Mechanisms in Chloroplasts of Alkaligrass Revealed by Phosphoproteomics.

Suo Jinwei J   Zhang Heng H   Zhao Qi Q   Zhang Nan N   Zhang Yongxue Y   Li Ying Y   Song Baohua B   Yu Juanjuan J   Cao Jianguo J   Wang Tai T   Luo Ji J   Guo Lihai L   Ma Jun J   Zhang Xumin X   She Yimin Y   Peng Lianwei L   Ma Weimin W   Guo Siyi S   Miao Yuchen Y   Chen Sixue S   Qin Zhi Z   Dai Shaojun S  

Genomics, proteomics & bioinformatics 20200601 3


Alkali-salinity exerts severe osmotic, ionic, and high-pH stresses to plants. To understand the alkali-salinity responsive mechanisms underlying photosynthetic modulation and reactive oxygen species (ROS) homeostasis, physiological and diverse quantitative proteomics analyses of alkaligrass (Puccinellia tenuiflora) under Na<sub>2</sub>CO<sub>3</sub> stress were conducted. In addition, Western blot, real-time PCR, and transgenic techniques were applied to validate the proteomic results and test t  ...[more]

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