Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap Elite
ORGANISM(S): Rattus Norvegicus (rat)
TISSUE(S): Brain, Neuron Of Cerebral Cortex, Cell Culture
SUBMITTER: Chih-Fan Chuang
LAB HEAD: Yen-Chung Chang
PROVIDER: PXD005527 | Pride | 2018-04-17
REPOSITORIES: Pride
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MaxQuant_1.5.2.8.zip | Other | |||
PeakList-1stLabeling.zip | Other | |||
PeakList-1stSwap.zip | Other | |||
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PeakList-2ndSwap.zip | Other |
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Chuang Chih-Fan CF King Chih-En CE Ho Bo-Wei BW Chien Kun-Yi KY Chang Yen-Chung YC
Journal of proteome research 20180419 5
The axon is a long projection connecting a neuron to its targets. Here, the axons of cultured rat cortical neurons were isolated with micropatterned chips that enable the separation of axons from their cell bodies. Proteins extracted from isolated axons and whole neurons were subjected to analyses using two-dimensional liquid chromatography-tandem mass spectrometry (2D-LC-MS/MS) analyses without and with stable isotope dimethyl labeling, resulting in the identification of >2500 axonal proteins a ...[more]