Proteomics

Dataset Information

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Unbiased proteomic study of the axons of cultured rat cortical neurons


ABSTRACT: We have conducted quantitative proteomic analyses of the axons of cultured rat cortical neurons. Axons are isolated by using glass chips that enable the axons and their cell bodies of neurons to grow in separated regions on the chip surface. Proteins extracted from the isolated axons, as well as those extracted from whole cortical neurons are subjected to two-dimensional liquid chromatography (2D-LC)-mass spectrometry (MS)-MS analyses. The abundances of proteins in the axon are found to be strongly correlated with their average abundances in whole neurons. Based upon these data, a quantitative description of the protein distribution among various subcellular structures in the axon has been generated. The proteins extracted from the axons and whole neurons are also subjected to stable isotope dimethyl labeling reaction and then to 2D-LC-MS/MS analysis. Proteins enriched in the axon compartment of rat cortical neurons are thus identified.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Brain, Neuron Of Cerebral Cortex, Cell Culture

SUBMITTER: Chih-Fan Chuang  

LAB HEAD: Yen-Chung Chang

PROVIDER: PXD005527 | Pride | 2018-04-17

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
MaxQuant_1.5.2.8.zip Other
PeakList-1stLabeling.zip Other
PeakList-1stSwap.zip Other
PeakList-2ndLabeling.zip Other
PeakList-2ndSwap.zip Other
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Publications

Unbiased Proteomic Study of the Axons of Cultured Rat Cortical Neurons.

Chuang Chih-Fan CF   King Chih-En CE   Ho Bo-Wei BW   Chien Kun-Yi KY   Chang Yen-Chung YC  

Journal of proteome research 20180419 5


The axon is a long projection connecting a neuron to its targets. Here, the axons of cultured rat cortical neurons were isolated with micropatterned chips that enable the separation of axons from their cell bodies. Proteins extracted from isolated axons and whole neurons were subjected to analyses using two-dimensional liquid chromatography-tandem mass spectrometry (2D-LC-MS/MS) analyses without and with stable isotope dimethyl labeling, resulting in the identification of >2500 axonal proteins a  ...[more]

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