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Regulated local synthesis of ribosomal proteins maintains ribosome function in axons

ABSTRACT: Ribosome assembly occurs mainly in the nucleolus yet recent studies have revealed robust enrichment and translation of mRNAs encoding many ribosomal proteins (RPs) in axons, far away from neuronal cell bodies. Here, we report a physical and functional interaction between locally synthesized RPs and ribosomes in the axon. We show that axonal RP translation is regulated through a novel sequence motif, CUIC, that forms an RNA-loop structure in the region immediately upstream of the initiation codon. Using imaging and subcellular proteomics techniques, we show that RPs synthesized in axons join axonal ribosomes in a nucleolus-independent fashion. Inhibition of axonal CUIC-regulated RP translation causes a significant decline in local translation activity and markedly reduces axon branching in the brain, revealing the physiological relevance of axonal RP synthesis in vivo. These results suggest that axonal translation supplies cytoplasmic RPs to maintain/modify local ribosomal function far from the nucleolus in neurons.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Xenopus Laevis (african Clawed Frog)

TISSUE(S): Brain, Retinal Ganglion Cell

SUBMITTER: Toshiaki Shigeoka

LAB HEAD: Christine E Holt

PROVIDER: PXD015574 | Pride | 2019-12-09

REPOSITORIES: Pride

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			Action	DRS
	P468_1a.raw	Raw
	P468_1b.raw	Raw
	P468_1c.raw	Raw
	P468_2a.raw	Raw
	P468_2b.raw	Raw

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