Dataset Information

N-Glycoproteomic Analysis of Botryococcus Braunii

ABSTRACT: We have performed an N-glycoproteomic analysis of the green microalgae Botryococcus braunii, a promising candidate for the production of biofuels, accumulating considerable amounts of hydrocarbon oils. Thereby, three different strains have been compared: Showa (Race B), AC761 (Race B) and CCALA778 (Race A) which differ in the type of produced hydrocarbons. In total, 517 unique N-glycosylated peptides have been identified analyzing intact N-glycopeptides as well as deglycosylated, 18O-labeled peptides. Intact N-glycopeptides that harbored N-acetylhexosamine (HexNAc) at the non-reducing end were identified. Surprisingly, these GnTI-dependent N-glycans were also found to be modified with (di)methylated hexose. This type of methylated GnTI-dependent N-glycans has not been described so far.

INSTRUMENT(S): LTQ Orbitrap, Q Exactive

ORGANISM(S): Botryococcus Braunii

TISSUE(S): Photosynthetic Cell

SUBMITTER: Stefan Schulze

LAB HEAD: Michael Hippler

PROVIDER: PXD005708 | Pride | 2017-08-08

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	AC761_20160205_proteins_target_decoy.fasta	Fasta
	CCALA778_proteins_target_decoy.fasta	Fasta
	Glyco_all_18O_results_merged.csv	Csv
	Glyco_all_18O_results_merged.u.json.u.json	Other
	Glyco_all_ISCID_results_N-HexNAc_merged.csv	Csv

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Publications

Identification of methylated GnTI-dependent N-glycans in Botryococcus brauni.

Schulze Stefan S Urzica Eugen E Reijnders Maarten J M F MJMF van de Geest Henri H Warris Sven S Bakker Linda V LV Fufezan Christian C Martins Dos Santos Vitor A P VAP Schaap Peter J PJ Peters Sander A SA Hippler Michael M

The New phytologist 20170724 4

In contrast to mammals and vascular plants, microalgae show a high diversity in the N-glycan structures of complex N-glycoproteins. Although homologues for β1,2-N-acetylglucosaminyltransferase I (GnTI), a key enzyme in the formation of complex N-glycans, have been identified in several algal species, GnTI-dependent N-glycans have not been detected so far. We have performed an N-glycoproteomic analysis of the hydrocarbon oils accumulating green microalgae Botryococcus braunii. Thereby, the analys ...[more]

PMID: 28737213

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