Proteomics

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A quantitative proteomic profile revealed the crucial roles of lysosome in the microbial infection

ABSTRACT: Lysosome played vital roles for both innate and adaptive immunity. It has been widely accepted that lysosome functioned more than a digestive organelle. However, it was still largely unknown for the proteome changes of lysosome on various microbes, and the understanding of lysosomal proteome from the purified lysosome is another obstacle that needs to be resolved. Here, we performed a proteomic study on the enriched lysosomes from THP1 cells after infected by Listeria monocytogenes (L. m), Herpes Simplex Virus 1 (HSV-1) and Vesicular Stomatitis Virus (VSV). Combining with the GO analysis, we identified 284 lysosomal related proteins from the totally identified 4560 proteins. We also constructed the protein-protein interaction networks for the changed proteins and revealed the core lysosomal proteins in responding for diverse microbial infections. From this study, we not only revealed that lysosome responded in a different manner for the bacterial or virus infection, but also proposed a new way for the proteome research for the enriched organelles.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Wei Ge  

LAB HEAD: Wei Ge

PROVIDER: PXD005748 | Pride | 2017-05-04

REPOSITORIES: pride

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Quantitative proteomic profiling for clarification of the crucial roles of lysosomes in microbial infections.

Xu Benhong B   Gao Yanpan Y   Zhan Shaohua S   Ge Wei W  

Molecular immunology 20170425

Lysosomes play vital roles in both innate and adaptive immunity. It is widely accepted that lysosomes do not function exclusively as a digestive organelle. It is also involved in the process of immune cells against pathogens. However, the changes in the lysosomal proteome caused by infection with various microbes are still largely unknown, and our understanding of the proteome of the purified lysosome is another obstacle that needs to be resolved. Here, we performed a proteomic study on lysosome  ...[more]

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