Dataset Information

Shotgun Proteomic Analysis of Proteins Present in Mosquito Ovaries at 24 and 72 h After a Blood Meal

ABSTRACT: Female mosquitoes require a blood meal for oogenesis, and thereby receive a substantial iron load in the forms of holo-transferrin and hemoglobin. Our previous data showed that during digestion of a blood meal, the gut iron concentration decreases 10-fold, while that of the ovaries doubles from ingestion to 72 hours post feeding. Approximately 72 hours post feeding, eggs are laid with ~125 ng Fe each. We are interested in the effects of the blood meal on the expression of iron related proteins detected in the ovaries during time post feeding before eggs are laid. We have used shotgun proteomic analysis to identify proteins in the developing ovaries of Aedes aegypti; this information provides further insight into the effect of a blood meal on mosquito oogenesis.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Aedes Aegypti

TISSUE(S): Ovary

SUBMITTER: Dawn Geiser

LAB HEAD: Joy J. Winzerling

PROVIDER: PXD005893 | Pride | 2022-03-26

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	Mudpit_WL_212164_C1.mzid.gz	Mzid
	Mudpit_WL_212164_C1.mzid_Mudpit_WL_212164_C1.MGF	Mzid
	Mudpit_WL_212164_C1.mzid_Mudpit_WL_212164_C1.pride.mgf.gz	Mzid
	Mudpit_WL_212164_C1.pride.mztab.gz	Mztab
	Mudpit_WL_212164_F1.mzid.gz	Mzid

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Publications

Shotgun and TMT-Labeled Proteomic Analysis of the Ovarian Proteins of an Insect Vector, Aedes aegypti (Diptera: Culicidae).

Geiser Dawn L DL Li Wenzhou W Pham Daphne Q-D DQ Wysocki Vicki H VH Winzerling Joy J JJ

Journal of insect science (Online) 20220301 2

Aedes aegypti [Linnaeus in Hasselquist; yellow fever mosquito] transmits several viruses that infect millions of people each year, including Zika, dengue, yellow fever, chikungunya, and West Nile. Pathogen transmission occurs during blood feeding. Only the females blood feed as they require a bloodmeal for oogenesis; in the bloodmeal, holo-transferrin and hemoglobin provide the females with a high iron load. We are interested in the effects of the bloodmeal on the expression of iron-associated p ...[more]

PMID: 35303100

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