Dataset Information

Gel-free/label-free proteomic, biochemical and photosynthetic anaysis of cowpea resistance against Cowpea severe mosaic virus

ABSTRACT: In this present work, photosynthetic and biochemical parameters in conjunction with gel-free/label-free proteomic analysis were assessed in a resistant cowpea genotype challenged with CPSMV and compared with the corresponding data of the mock-inoculated control as an attempt to find out some clues to the molecular mechanims by which this cowpea genotype is resistant to CPSMV.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Vigna Unguiculata

TISSUE(S): Plant Cell, Leaf

DISEASE(S): Wounds And Injuries

SUBMITTER: Wang Xin

LAB HEAD: Jose T. A. Oliveira

PROVIDER: PXD005906 | Pride | 2024-09-12

REPOSITORIES: pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 40

Publications

Gel-free/label-free proteomic, photosynthetic, and biochemical analysis of cowpea (Vigna unguiculata [L.] Walp.) resistance against Cowpea severe mosaic virus (CPSMV).

Varela Anna Lidia N ALN Komatsu Setsuko S Wang Xin X Silva Rodolpho G G RGG Souza Pedro Filho N PFN Lobo Ana Karla M AKM Vasconcelos Ilka M IM Silveira Joaquim A G JAG Oliveira Jose T A JTA

Journal of proteomics 20170511

Cowpea severe mosaic virus (CPSMV) causes significant losses in cowpea (Vigna unguiculata) production. In this present study biochemical, physiological, and proteomic analysis were done to identify pathways and defense proteins that are altered during the incompatible interaction between the cowpea genotype BRS-Marataoã and CPSMV. The leaf protein extracts from mock- (MI) and CPSMV-inoculated plantlets (V) were evaluated at 2 and 6days post-inoculation (DPI). Data support the assumptions that in ...[more]

PMID: 28502737

Similar Datasets

Project description:Grapevine red blotch is a recently identified viral disease that was first recognized in the Napa Valley of California. Infected plants showed foliar symptoms similar to leafroll, another grapevine viral disease, on vines testing negative for known grapevine leafroll-associated virus. Later, the Grapevine red blotch virus (GRBV) was independently discovered in the US states of California and New York and was demonstrated to be the causal agent of red blotch disease. Due to its wide occurrence in the US, vector transmission and impacts on grape industry, this virus has the potential to cause serious economic losses. Despite numerous attempts, it was not possible to isolate or visualize viral particles from GRBV infected plants. Consequently, this has hampered the development of a serological assay that would facilitate GRBV detection in grapevine. We therefore decided to explore mass spectrometry approaches in order to quantify GRBV in infected plants and to identify potential biomarkers for viral infection. We present for the first time the physical detection on the protein level of the two GRBV genes V1 (coat protein) and V2 in grapevine tissue lysates. The GRBV coat protein load in leaf petioles was determined to be in the range of 100 to 900 million copies per milligram wet weight by using three heavy isotope labeled reference peptides as internal standards. The V1 copy number per unit wet tissue weight in leaves appeared to be about six times lower, and about 200-times lower in terms of protein concentration in the extractable protein mass than in petioles. We found a consistent upregulation of several enzymes involved in flavonoid biosynthesis in leaf and petiole extracts of GRBV-infected plants by label-free shotgun proteomics, indicating the activation of a defense mechanism against GRBV, a plant response already described for grapevine leafroll associated virus infection on the transcriptome level. Last but not least, we identified some other microorganisms belonging to the grapevine leaf microbiota, two bacterial species (Novosphingobium sp. Rr 2-17 and Methylobacterium) and one virus, Grapevine rupestris stem pitting associated virus.

			Action	DRS
	R1I2.mgf	Mgf
	R1I2.msf	Msf
	R1I2.pep.xml	Pepxml
	R1I2.raw	Raw
	R1I6.mgf	Mgf