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Proteomic identification of rainbow trout blood plasma proteins and their relationship to seminal plasma proteins

ABSTRACT: The characterization of fish blood proteomes is important for the comparative studies of seminal and blood proteins as well as for the analysis of fish immune mechanisms and pathways. In this study LC-MS/MS and 2D DIGE was applied to compare seminal and blood plasma trout proteomes. The 54 differentially abundant proteins identified in seminal plasma are involved in a variety of signaling pathways, including protein ubiqutination, liver X receptor/retinoid X receptor (LXR/RXR) and farnesoid X (FXR)/RXR activation, cell cycle and acute phase signaling. These finding may indicate the prevalence of acute phase signaling pathways in trout seminal plasma, and its essential role in protecting spermatozoa and reproductive tissues. Furthermore our study provides the first in-depth analysis of the rainbow trout blood plasma proteome, with a total of 119 identified proteins. The major proteins of rainbow trout blood plasma were recognized as acute phase proteins. The Ingenuity Pathway Analysis of trout BP plasma proteins indicated that acute phase response signaling, complement system, LXR, FXR-RXR activation and coagulation system are the top canonical pathways associated with trout blood proteins. In conclusion this study enhances the knowledge of the blood origin of trout seminal plasma proteins and the understanding of fish reproductive biology. Additionally our results provide new insight into blood proteins specifically important for fish physiology and innate immunity.

INSTRUMENT(S): LTQ

ORGANISM(S): Oncorhynchus Mykiss (rainbow Trout) (salmo Gairdneri)

TISSUE(S): Blood Cell, Blood Plasma

SUBMITTER: Joanna Nynca

LAB HEAD: Andrzej Ciereszko

PROVIDER: PXD005988 | Pride | 2017-12-11

REPOSITORIES: Pride

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Proteomic identification of rainbow trout blood plasma proteins and their relationship to seminal plasma proteins.

Nynca Joanna J Arnold Georg G Fröhlich Thomas T Ciereszko Andrzej A

Proteomics 20170601 11

The characterisation of fish blood proteomes is important for comparative studies of seminal and blood proteins as well as for the analysis of fish immune mechanisms and pathways. In this study, LC-MS/MS and 2D-DIGE were applied to compare rainbow trout seminal (SP) and blood plasma (BP) proteomes. The 54 differentially abundant proteins identified in SP are involved in a variety of signalling pathways, including protein ubiquitination, liver X receptor/retinoid X receptor (LXR/RXR) and farnesoi ...[more]

PMID: 28394474

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Project description:Triptolide (TP), the major active component in Tripterygium wilfordii Hook. f. (Tripterygium Glycosides), contributes to treat rheumatoid arthritis and anticancer activities. However, the organ toxicity, especially nephrotoxicity and hepatotoxicity limit its clinical application. To fully understand the mechanism underlying the triptolide induced toxicity, iTRAQ based proteomics and targeted fatty acids analysis were performed. In the present study, mouse were treated with LD50 dose of triptolide, and plasma, liver and kidney tissues were harvested before drug administration (0 h) and after drug administration (0.5 h, 1 h, 2 h, 8 h, 16 h). The blood biochemical levels (ALT, AST, BUN and CRE) were used to evaluated the toxicity of triptolide. 2D-LC-MS/MS approach was used to compare different proteins among groups 0h, 1h, 2h and 8 h. Functional annotation of different proteins in liver between different groups reveals that the top 3 pathways influenced by the TP were acute phase response signaling, antigen presentation pathway and FXR/RXR activation, the molecular and cellular functions which was mainly effected were lipid metabolism and small molecule biochemistry. In kidney, the pathway including LPS/IL-1 Mediated Inhibition of RXR Function, EIF2 Signaling, acute phase response signaling, and LXR/RXR Activation were mainly affected. The proteomics data implicated that fatty acids (FAs) may involve in the organ toxicity induced by TP. Then targeted fatty acids analysis was carried out to determinate the concentration between the different groups (0.5 h, 1 h, 2 h, 8 h, 16 h) by HPLC-MRM. We found that fatty acids in liver (C17:0, C18:0, C18:2, C18:3, C20:0, C20:3, C20:4, C22:1, C22:2, C22:3, C22:4, C22:5, C22:6, C24:0, C24:1, C24:2, C24:3, C24:4, C24:5, C24:6) show significant difference among groups, while in kidney, FAs (C12:0, C12:1, C14:0, C14:1, C16:1, C16:2) show significant difference. P450 protein family show significant change in different group, protein CYP4A14 demonstrate change in both kidney and liver tissues. By combing proteomics and targeted FAs analysis, we deeply investigated the mechanism underlying the TP induced organ toxicity, and the results may provide deeply insight into prevention or intervention in the TP clinical usage and improving its safety.

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	BP1Band01.RAW	Raw
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	BP1Band05.RAW	Raw