Dataset Information

Extracellular matrix downregulation in the Drosophila heart preserves contractile function and improves lifespan

ABSTRACT: Aging is associated with extensive remodeling of the heart, including basement membrane (BM) components that surround cardiomyocytes. Remodeling is thought to impair cardiac mechanotransduction, but the contribution of specific BM components to age-related lateral communication between cardiomyocytes is unclear. Using a genetically tractable, rapidly aging model with sufficient cardiac genetic homology and morphology, e.g. Drosophila melanogaster, we observed differential regulation of BM collagens between laboratory strains, correlating with changes in muscle physiology leading to cardiac dysfunction. Therefore, we sought to understand the extent to which BM proteins modulate contractile function during aging. Cardiac-restricted knockdown of ECM genes Pericardin, Laminin A, and Viking in Drosophila prevented age-associated heart tube restriction and increased contractility, even under viscous load. Most notably, reduction of Laminin A expression correlated with an overall preservation of contractile velocity with age and extension of organismal lifespan. Global heterozygous knockdown confirmed these data, which provides new evidence of a direct link between BM homeostasis, contractility, and maintenance of lifespan.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Heart

SUBMITTER: Koen Raedschelders

LAB HEAD: Jennifer Van Eyk

PROVIDER: PXD006120 | Pride | 2017-03-27

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	KR_150601_Elite_W1118_1wk_2-11-15.pep.xml	Pepxml
	KR_150601_Elite_W1118_1wk_2-11-15.raw	Raw
	KR_150601_Elite_W1118_1wk_2-17-15.pep.xml	Pepxml
	KR_150601_Elite_W1118_1wk_2-17-15.raw	Raw
	KR_150601_Elite_W1118_1wk_2-26-15.pep.xml	Pepxml

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Extracellular matrix downregulation in the Drosophila heart preserves contractile function and improves lifespan.

Sessions Ayla O AO Kaushik Gaurav G Parker Sarah S Raedschelders Koen K Bodmer Rolf R Van Eyk Jennifer E JE Engler Adam J AJ

Matrix biology : journal of the International Society for Matrix Biology 20161025

Aging is associated with extensive remodeling of the heart, including basement membrane (BM) components that surround cardiomyocytes. Remodeling is thought to impair cardiac mechanotransduction, but the contribution of specific BM components to age-related lateral communication between cardiomyocytes is unclear. Using a genetically tractable, rapidly aging model with sufficient cardiac genetic homology and morphology, e.g. Drosophila melanogaster, we observed differential regulation of BM collag ...[more]

PMID: 27793636

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