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Exploring the potential role of moonlighting function of the surface-associated proteins from Mycobacterium bovis BCG Moreau and Pasteur by comparative proteomic

ABSTRACT: Tuberculosis (TB) is one of major causes of death worldwide. Bacillus Calmette-Guerin (BCG) is the only licensed TB vaccine and its inability to protect against adult pulmonary TB can be due to genetic differences among strains described since the 1940s. In this work, we compared the proteomic profile of the surface-associated proteins from M. bovis BCG Moreau, the Brazilian vaccine strain, and the BCG Pasteur reference strain. The methodology used was 2D-gel electrophoresis combined with mass spectrometry techniques (MALDI-TOF/TOF). We identified 115 proteins. Of these, 24 proteins showed differential expression between the two BCG strains. Furthermore, 27 proteins previously described as displaying moonlighting function were identified, 8 of these proteins showed variation in abundance comparing BCG Moreau to Pasteur and 2 of them presented two different domain hits.

INSTRUMENT(S): 4800 Proteomics Analyzer

ORGANISM(S): Mycobacterium Bovis Bcg Str. Moreau Rdj

SUBMITTER: Dario E. Kalume

LAB HEAD: Da¡rio Eluan Kalume

PROVIDER: PXD006141 | Pride | 2019-05-15

REPOSITORIES: Pride

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Exploring the Potential Role of Moonlighting Function of the Surface-Associated Proteins From <i>Mycobacterium bovis</i> BCG Moreau and Pasteur by Comparative Proteomic.

Pagani Talita Duarte TD Guimarães Ana Carolina R ACR Waghabi Mariana C MC Corrêa Paloma Rezende PR Kalume Dário Eluan DE Berrêdo-Pinho Marcia M Degrave Wim Maurits WM Mendonça-Lima Leila L

Frontiers in immunology 20190426

Surface-associated proteins from <i>Mycobacterium bovis</i> BCG Moreau RDJ are important components of the live Brazilian vaccine against tuberculosis. They are important targets during initial BCG vaccine stimulation and modulation of the host's immune response, especially in the bacterial-host interaction. These proteins might also be involved in cellular communication, chemical response to the environment, pathogenesis processes through mobility, colonization, and adherence to the host cell, ...[more]

PMID: 31080447

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			Action	DRS
	BCGMOREAU.txt	Txt
	ProteomicmapBCGMoreau.tif	Other
	RAWt2d.zip	Other
	Rv0009SPOT117pep.xml	Xml
	Rv0009SPOT67pep.xml	Xml