Dataset Information

Comparison of control and Elmod1 knockout utricle extracts using tandem mass tagging

ABSTRACT: Elmod1 knockout mice develop a severe inner ear defect beginning at early postnatal ages. Cuticular plates of utricle hair cells were initially formed normally, then degenerated after postnatal day 5 (P5). Similarly, hair bundles initially were formed normally, but the cell’s apical membrane subsequently lifted up; fused stereocilia were observed after P5. We used tandem mass tagging to determine whether there were any changes in medium- to high-abundance proteins in mutant animals. No statistically significant differences were seen in any protein quantified with two or more peptides.

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Utricle

SUBMITTER: Peter Barr-Gillespie

LAB HEAD: Peter Barr-Gillespie

PROVIDER: PXD006164 | Pride | 2018-10-24

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	GILL-125_MouseEarUtricle_HET_KO_Normalization_0pt8ug_25cm_120min_uLPickup_20151012_OT.raw	Raw
	GILL-125_MouseEarUtricles_HETandKO_TMT_10pt4ug_14pctACN_25cm_120min_20151024_OT.raw	Raw
	GILL-125_MouseEarUtricles_HETandKO_TMT_10pt4ug_20pctACN_25cm_120min_20151024_OT.raw	Raw
	GILL-125_MouseEarUtricles_HETandKO_TMT_10pt4ug_24pctACN_25cm_120min_20151024_OT.raw	Raw
	GILL-125_MouseEarUtricles_HETandKO_TMT_10pt4ug_28pctACN_25cm_120min_20151024_OT.raw	Raw

Items per page:

1 - 5 of 13

Publications

ELMOD1 Stimulates ARF6-GTP Hydrolysis to Stabilize Apical Structures in Developing Vestibular Hair Cells.

Krey Jocelyn F JF Dumont Rachel A RA Wilmarth Philip A PA David Larry L LL Johnson Kenneth R KR Barr-Gillespie Peter G PG

The Journal of neuroscience : the official journal of the Society for Neuroscience 20171208 4

Sensory hair cells require control of physical properties of their apical plasma membranes for normal development and function. Members of the ADP-ribosylation factor (ARF) small GTPase family regulate membrane trafficking and cytoskeletal assembly in many cells. We identified ELMO domain-containing protein 1 (ELMOD1), a guanine nucleoside triphosphatase activating protein (GAP) for ARF6, as the most highly enriched ARF regulator in hair cells. To characterize ELMOD1 control of trafficking, we a ...[more]

PMID: 29222402

Similar Datasets

Project description:Sweat glands play a fundamental role in thermal regulation in man, but the molecular mechanism of their development remains unknown. To initiate analyses, we compared the model of Eda mutant Tabby mice, in which sweat glands were not formed, to wild-type mice. We inferred developmental stages and critical genes based on observations at 7 time points spanning embryonic, postnatal and adult life. In wild-type footpads, sweat gland germs were detected at E17.5. The coiling of secretory portions started at postnatal day 1 (P1), and sweat gland formation was essentially complete by P5. Consistent with a controlled morphological progression, expression profiling revealed stage-specific gene expression changes. Similar to the development of hair follicles the other major skin appendage controlled by EDA sweat gland induction and initial progression was accompanied by Eda-dependent up-regulation of the Shh pathway. During the further development of sweat gland secretory portions, Foxa1 and Foxi1, not at all expressed in hair follicles, were progressively up-regulated in wild-type but not in Tabby footpads. Upon completion of wild-type development, Shh declined to Tabby levels, but Fox family genes remained at elevated levels in mature sweat glands. The results provide a framework for the further analysis of phased downstream regulation of gene action, possibly by a signaling cascade, in response to Eda. This SuperSeries is composed of the following subset Series: GSE14870: Requirement for Shh and Fox family genes at different stages in sweat gland development (E15.5) GSE14871: Requirement for Shh and Fox family genes at different stages in sweat gland development (E16.5) GSE14872: Requirement for Shh and Fox family genes at different stages in sweat gland development (E17.5) GSE14873: Requirement for Shh and Fox family genes at different stages in sweat gland development (P1) GSE14874: Requirement for Shh and Fox family genes at different stages in sweat gland development (P3) GSE14876: Requirement for Shh and Fox family genes at different stages in sweat gland development (P5) GSE14877: Requirement for Shh and Fox family genes at different stages in sweat gland development (Adult) To define target genes of Eda during sweat gland development, we carried out microarray experiments with mouse footpads that from 7 developmental time points including E15.5, E16.5, E17.5, P1, P3, P5 and 8 weeks of wild-type and Tabby mice. Refer to individual Series