Dataset Information

Proteomics analysis of wheat flag leaf in heat stress

ABSTRACT: Wheat plants (T. aestivum cv. Chinese Spring) were grown in a greenhouse and watered daily to avoid drought stress. The main stem ears were labelled when flowering. At 12 days after anthesis, the plants were transferred to growth chambers with a temperature regime of 24/17◦C (14 h/10 h) day/night cycle. At 15 days after anthesis, half of the plants were applied a temperature regime of 37/17◦C for 3 days and were harvested on the 4th day after 4 h under heat with light; the remaining plants were kept at 24/17◦C as a control and were harvested at the same sampling time.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Triticum Aestivum (wheat)

TISSUE(S): Flag Leaf

SUBMITTER: Yunze Lu

LAB HEAD: Shengbao Xu

PROVIDER: PXD006207 | Pride | 2017-07-07

REPOSITORIES: Pride

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Publications

Comparative Proteomic Analysis of Flag Leaves Reveals New Insight into Wheat Heat Adaptation.

Lu Yunze Y Li Ruiqiong R Wang Ruochen R Wang Xiaoming X Zheng Weijun W Sun Qixin Q Tong Shaoming S Dai Shaojun S Xu Shengbao S

Frontiers in plant science 20170620

Hexaploid wheat (<i>Triticum aestivum</i> L.) is an important food crop but it is vulnerable to heat. The heat-responsive proteome of wheat remains to be fully elucidated because of previous technical and genomic limitations, and this has hindered our understanding of the mechanisms of wheat heat adaptation and advances in improving thermotolerance. Here, flag leaves of wheat during grain filling stage were subjected to high daytime temperature stress, and 258 heat-responsive proteins (HRPs) wer ...[more]

PMID: 28676819

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Project description:Effects of heat priming applied to the first generation on tolerance of the successive generation to post-anthesis high temperature stress were investigated. Compared with the progeny of non-heat primed plants (NH), the progeny of heat-primed plants (PH) presented higher grain yield, leaf photosynthesis and activities of antioxidant enzymes and lower cell membrane damage under high temperature stress. In the transcriptome profile, 1430 probes showed obvious difference in expression between PH and NH. These genes were those of signal transduction, transcription, energy, defense, and protein destination and storage, respectively. Pot experiments (25 cm in diameter and 22 cm in depth) were conducted at the Experimental Station of Nanjing Agricultural University, Nanjing, China (32Ë30Ê¹N and 118Ë42Ê¹E). During the first generation, wheat plants were divided into two groups: one group were not primed (N) while the other were heat-primed (P) at pre-anthesis (at a day/night temperature of 32/28ËC for two days at the seven-leaf stage and the nine-leaf stage) and post-anthesis (34/30ËC for seven days at the 10th day after anthesis). At maturity, seeds from both groups were harvested. Seedlings (the progeny generation) from each group seed were further divided two sub-groups: one was subjected to high temperature stress at a day/night temperature of 34/30ËC, while the other was set at 26/22ËC for six days from the 10th day after anthesis (DAA). During both priming and high-temperature stress, plants under different treatments were moved into sepatarate growth chambers at preset temperatures. Thereafter, four treatments were established: NC, progeny of non-primed plants without post-anthesis high temperature stress; NH, progeny of non-primed plants with post-anthesis high temperature stress; PC, progeny of primed plants without post-anthesis high temperature stress; and PH, progeny of primed plants with post-anthesis high temperature stress.

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Dataset Information

Proteomics analysis of wheat flag leaf in heat stress

Dataset's files

Publications

Comparative Proteomic Analysis of Flag Leaves Reveals New Insight into Wheat Heat Adaptation.

Similar Datasets

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