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Hydrophobic interaction chromatography for bottom-up proteomics

ABSTRACT: Hydrophobic interaction chromatography as a first dimension to reduce samples complexity for bottom-up proteomics analyses of single proteins and protein complexes

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Colon Cancer

SUBMITTER: Joern Dengjel

LAB HEAD: Joern Dengjel

PROVIDER: PXD006217 | Pride | 2017-05-16

REPOSITORIES: Pride

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			Action	DRS
	20151204_MR_Exp82_1.RAW	Raw
	20151204_MR_Exp82_10.RAW	Raw
	20151204_MR_Exp82_2.RAW	Raw
	20151204_MR_Exp82_3.RAW	Raw
	20151204_MR_Exp82_4.RAW	Raw

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Hydrophobic Interaction Chromatography for Bottom-Up Proteomics Analysis of Single Proteins and Protein Complexes.

Rackiewicz Michal M Große-Hovest Ludger L Alpert Andrew J AJ Zarei Mostafa M Dengjel Jörn J

Journal of proteome research 20170511 6

Hydrophobic interaction chromatography (HIC) is a robust standard analytical method to purify proteins while preserving their biological activity. It is widely used to study post-translational modifications of proteins and drug-protein interactions. In the current manuscript we employed HIC to separate proteins, followed by bottom-up LC-MS/MS experiments. We used this approach to fractionate antibody species followed by comprehensive peptide mapping as well as to study protein complexes in human ...[more]

PMID: 28485144

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