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Accumulated ion monitoring for improved sensitivity

ABSTRACT: This work aimed to improve sensitivity of targeted detection by orbitrap mass spectrometers. Co-isolation of contaminant ions was identified as the major factor limiting sensitivity, and LOD of both PRM and accumulated precursor ion scanning (AIM) was improved by increased chromatographic resolution.

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Suspension Culture

SUBMITTER: Alex Kentsis

LAB HEAD: Alex Kentsis

PROVIDER: PXD006236 | Pride | 2017-08-24

REPOSITORIES: Pride

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			Action	DRS
	150122_PC_AIM3_new_tip_FT240_SAMPLE0.raw	Raw
	150122_PC_AIM3_new_tip_FT240_SAMPLE01.raw	Raw
	150122_PC_AIM3_new_tip_FT240_SAMPLE02.raw	Raw
	150122_PC_AIM3_new_tip_FT240_SAMPLE03.raw	Raw
	150122_PC_AIM3_new_tip_FT240_SAMPLE04.raw	Raw

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Publications

High Sensitivity Quantitative Proteomics Using Automated Multidimensional Nano-flow Chromatography and Accumulated Ion Monitoring on Quadrupole-Orbitrap-Linear Ion Trap Mass Spectrometer.

Cifani Paolo P Kentsis Alex A

Molecular & cellular proteomics : MCP 20170818 11

Quantitative proteomics using high-resolution and accuracy mass spectrometry promises to transform our understanding of biological systems and disease. Recent development of parallel reaction monitoring (PRM) using hybrid instruments substantially improved the specificity of targeted mass spectrometry. Combined with high-efficiency ion trapping, this approach also provided significant improvements in sensitivity. Here, we investigated the effects of ion isolation and accumulation on the sensitiv ...[more]

PMID: 28821601

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