Proteomics

Dataset Information

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Improvements on the quantitative analysis of Trypanosoma cruzi histone post translational modifications


ABSTRACT: Trypanosome histone N-terminal sequences are very divergent from the other eukaryotes, although they are still decorated by post-translational modifications (PTMs). Here, we used a highly robust workflow to analyze histone PTMs in the parasite Trypanosoma cruzi using mass spectrometry-based data-independent acquisition (DIA). We adapted the workflow for the analysis of the parasite’s histone sequences by modifying the software EpiProfile 2.0, improving peptide and PTM quantification accuracy. This workflow could now be applied to the study of 141 T. cruzi modified histone peptides, which we used to investigate the dynamics of histone PTMs along the metacyclogenesis and the life cycle of T. cruzi.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Trypanosoma Cruzi Cruzi

SUBMITTER: Julia Cunha  

LAB HEAD: Julia da Cunha

PROVIDER: PXD018295 | Pride | 2022-02-07

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
1histone_6.par Other
1histone_CIDIT_histonetest.mgf Mgf
1histone_F002262.dat Other
1histone_F002262.dat-pride.xml.gz Xml
1histone_Histone_HUMAN_mdb_comb_rever.fasta Fasta
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