Dataset Information

Fishing for SAM/SAH-binding proteins

ABSTRACT: The aim of the project was to identify S-adenodsyl methionine-binding proteins expressed in D. melanogaster S2 cells. For this puprose a capture compund reagent carrying a SAH-affinity group, a biotin group for enrichment and a cross-linking moiety consisting of either benzophenone or a nitrobenzene.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Cell Culture, Macrophage

SUBMITTER: Andreas Schmidt

LAB HEAD: Axel Imhof

PROVIDER: PXD006245 | Pride | 2019-02-25

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20140904_SV_benzophenone45uM_NE_1_5.wiff	Wiff
	20140904_SV_benzophenone45uM_NE_1_5.wiff.scan	Wiff
	20140904_SV_benzophenone45uM_NE_1_6.wiff	Wiff
	20140904_SV_benzophenone45uM_NE_1_6.wiff.scan	Wiff
	20140904_SV_benzophenone45uM_NE_1_7_2.wiff	Wiff

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Publications

Multi-Reference Spectral Library Yields Almost Complete Coverage of Heterogeneous LC-MS/MS Data Sets.

Ammar Constantin C Berchtold Evi E Csaba Gergely G Schmidt Andreas A Imhof Axel A Zimmer Ralf R

Journal of proteome research 20190308 4

Spectral libraries play a central role in the analysis of data-independent-acquisition (DIA) proteomics experiments. A main assumption in current spectral library tools is that a single characteristic intensity pattern (CIP) suffices to describe the fragmentation of a peptide in a particular charge state (peptide charge pair). However, we find that this is often not the case. We carry out a systematic evaluation of spectral variability over public repositories and in-house data sets. We show tha ...[more]

PMID: 30793903

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