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Proteome Analysis of Human Embryonic Stem Cells Organelles

ABSTRACT: Here, we have utilized a sub-cellular proteomics approach to analyze the localization of proteins in the nucleus, mitochondria, crude membrane, cytoplasm, heavy and light microsomes. Out of 2002 reproducibly identified proteins, we detected 762 proteins in a single organelle whereas 160 proteins were found in all sub-cellular fractions. We verified the localization of identified proteins through databases and discussed the consistency of the obtained results. With regards to the ambiguity in the definition of a membrane protein, we tried to clearly define the plasma membrane, peripheral membrane and membrane proteins by annotation of these proteins in databases, along with predictions of transmembrane helices.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Embryonic Stem Cell

SUBMITTER: Faezeh Shekari

LAB HEAD: Ghasem Hosseini Salekdeh

PROVIDER: PXD006271 | Pride | 2017-04-26

REPOSITORIES: pride

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			Action	DRS
	Crudemembranerep1.mgf	Mgf
	Crudemembranerep1.mzml	Mzml
	Crudemembranerep1.xml	Xml
	Crudemembranerep2.mgf	Mgf
	Crudemembranerep2.mzml	Mzml

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Publications

Proteome analysis of human embryonic stem cells organelles.

Shekari Faezeh F Nezari Hossein H Larijani Mehran Rezaei MR Han Chia-Li CL Baharvand Hossein H Chen Yu-Ju YJ Salekdeh Ghasem Hosseini GH

Journal of proteomics 20170420

As the functions of proteins are associated with their cellular localization, the comprehensive sub-cellular proteome knowledge of human embryonic stem cells (hESCs) is indispensable for ensuring a therapeutic effect. Here, we have utilized a sub-cellular proteomics approach to analyze the localization of proteins in the nucleus, mitochondria, crude membrane, cytoplasm, heavy and light microsomes. Out of 2002 reproducibly identified proteins, we detected 762 proteins in a single organelle wherea ...[more]

PMID: 28435121

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