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Competition-based, quantitative chemical proteomics in breast cancer cells identifies new target profiles for sulforaphane

ABSTRACT: Sulforaphane is a small molecule isothiocyanate which exhibits anticancer potential, yet its biological targets remain poorly understood. Here we employ a competition-based chemical proteomics strategy to profile sulforaphane’s targets and identify over 500 targets along with their relative affinities. These targets provide a new set of mediators for sulforaphane’s bioactivity, and aid understanding of its complex mode of action.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Thomas Lanyon-Hogg

LAB HEAD: Edward Tate

PROVIDER: PXD006279 | Pride | 2017-05-24

REPOSITORIES: Pride

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			Action	DRS
	HUMAN_REFERENCE_NOV_22_2016.fasta	Fasta
	JACB139.zip	Other
	JACB139_1.raw	Raw
	JACB139_10.raw	Raw
	JACB139_11.raw	Raw

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Competition-based, quantitative chemical proteomics in breast cancer cells identifies new target profiles for sulforaphane.

Clulow James A JA Storck Elisabeth M EM Lanyon-Hogg Thomas T Kalesh Karunakaran A KA Jones Lyn H LH Tate Edward W EW

Chemical communications (Cambridge, England) 20170501 37

Sulforaphane is a small molecule isothiocyanate which exhibits anticancer potential, yet its biological targets remain poorly understood. Here we employ a competition-based chemical proteomics strategy to profile sulforaphane's targets and identify over 500 targets along with their relative affinities. These targets provide a new set of mediators for sulforaphane's bioactivity, and aid understanding of its complex mode of action. ...[more]

PMID: 28439590

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