Dataset Information

A Temporal Proteomic Map of Epstein-Barr Virus Lytic Replication in B-cells

ABSTRACT: Epstein Barr virus (EBV) replication contributes to multiple human diseases, including infectious mononucleosis, nasopharyngeal carcinoma, B-cell lymphomas, and oral hairy leukoplakia. We performed systematic quantitative analyses of temporal changes in host and EBV proteins during lytic replication to gain novel insights into virus-host interactions, using conditional Burkitt lymphoma models of type I and II EBV infection. We quantified profiles of >8000 cellular and 69 EBV proteins, including >500 plasma membrane proteins, providing temporal views of the lytic B-cell proteome and EBV virome. Our approach revealed EBV-induced remodelling of cell cycle, innate and adaptive immune pathways, including upregulation of the complement cascade and proteasomal degradation of the B-cell receptor complex, conserved between EBV types I and II. Cross-comparison with proteomic analyses of human cytomegalovirus infection and of a Kaposi sarcoma associated herpesvirus immunoevasin identified host factors targeted by multiple herpesviruses. Our results provide an important resource for studies of EBV replication.

INSTRUMENT(S): Orbitrap Fusion Lumos, Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): B Cell, Cell Culture

DISEASE(S): Epstein-barr Virus Hepatitis

SUBMITTER: Michael Weekes

LAB HEAD: Michael Weekes

PROVIDER: PXD006317 | Pride | 2017-05-19

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	170305_EBV_Timecourse_Peptides.txt	Txt
	170305_EBV_Timecourse_Proteins.txt	Txt
	Akata_WCL_1702_a04642.raw	Raw
	Akata_WCL_1702_a04643.raw	Raw
	Akata_WCL_1702_a04644.raw	Raw

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Publications

A Temporal Proteomic Map of Epstein-Barr Virus Lytic Replication in B Cells.

Ersing Ina I Nobre Luis L Wang Liang Wei LW Soday Lior L Ma Yijie Y Paulo Joao A JA Narita Yohei Y Ashbaugh Camille W CW Jiang Chang C Grayson Nicholas E NE Kieff Elliott E Gygi Steven P SP Weekes Michael P MP Gewurz Benjamin E BE

Cell reports 20170501 7

Epstein-Barr virus (EBV) replication contributes to multiple human diseases, including infectious mononucleosis, nasopharyngeal carcinoma, B cell lymphomas, and oral hairy leukoplakia. We performed systematic quantitative analyses of temporal changes in host and EBV proteins during lytic replication to gain insights into virus-host interactions, using conditional Burkitt lymphoma models of type I and II EBV infection. We quantified profiles of >8,000 cellular and 69 EBV proteins, including >500 ...[more]

PMID: 28514666

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