Dataset Information

Identification of HLA-A11:01 and A02:01-restricted EBV Pep-tides using HLA Peptidomics

ABSTRACT: Identification of HLA-A*11:01 and A*02:01-restricted EBV Pep-tides using HLA Peptidomics

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Nasopharyngeal Carcinoma Cell, Cell Culture

SUBMITTER: yufei wang

LAB HEAD: Miao Xu

PROVIDER: PXD051538 | Pride | 2024-06-14

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	20231020_LM_SA_ZXF_SRN_XM_WYF_StrepIP_C01_1.raw	Raw
	20231020_LM_SA_ZXF_SRN_XM_WYF_StrepIP_C01_1_uncalibrated.mzML	Mzml
	20231020_LM_SA_ZXF_SRN_XM_WYF_StrepIP_C01_2.raw	Raw
	20231020_LM_SA_ZXF_SRN_XM_WYF_StrepIP_C01_2_uncalibrated.mzML	Mzml
	20231020_LM_SA_ZXF_SRN_XM_WYF_StrepIP_C02_1.raw	Raw

Items per page:

1 - 5 of 49

Publications

Identification of HLA-A11:01 and A02:01-Restricted EBV Peptides Using HLA Peptidomics.

Wang Yufei Y Zhang Wanlin W Shi Ruona R Luo Yanran Y Feng Zhenhuan Z Chen Yanhong Y Zhang Qiuting Q Zhou Yan Y Liang Jingtong J Ye Xiaoping X Feng Qisheng Q Zhang Xiaofei X Xu Miao M

Viruses 20240425 5

Epstein-Barr Virus (EBV) is closely linked to nasopharyngeal carcinoma (NPC), notably prevalent in southern China. Although type II latency of EBV plays a crucial role in the development of NPC, some lytic genes and intermittent reactivation are also critical for viral propagation and tumor progression. Since T cell-mediated immunity is effective in targeted killing of EBV-positive cells, it is important to identify EBV-derived peptides presented by highly prevalent human leukocyte antigen class ...[more]

PMID: 38793551

Similar Datasets

Project description:Human leukocyte antigen (HLA) - B*51:01 and endoplasmic reticulum aminopeptidase 1 (ERAP1) are two strong predisposing genetic factors to Behçet's disease (BD). Previous studies have focused on two subgroups of HLA-B*51 peptidome containing Proline (Pro) or Alanine (Ala) at position 2 (P2). Little is known about the unconventional non-Pro/Ala2 HLA-B*51-bound peptides. We aimed to study the features of this novel sub-peptidome, and investigate its regulation by ERAP1. CRISPR-Cas9 was used to generate an HLA-ABC-knockout HeLa cell line (HeLa.ABC-KO), which was subsequently transduced to express HLA-B*51:01 (HeLa.ABC-KO.B51). ERAP1 was silenced using lentiviral shRNA. Peptides bound to HLA-B*51:01 were eluted and analyzed by Mass Spectrometry. The characteristics of non-Pro/Ala2, Pro2 and Ala2 peptides, and their alteration by ERAP1 silencing were investigated. Effects of ERAP1 silencing on cell surface expression of HLA-B*51:01 were studied using flow cytometry. More than 20% of peptides eluted from HLA-B*51:01 lacked Proline or Alanine at P2. This unconventional group of HLA-B*51:01-bound peptides was enriched for 8-mers (with fewer 9-mers) compared to Pro2 and Ala2 sub-peptidomes, and had similar N-terminal and C-terminal residue usages to Ala2 peptides with the exception of the less abundant Leucine at position Ω. Knockdown of ERAP1 increased the percentage of non-Pro/Ala2 to approximately 40%, increased the percentage of longer (10-mer and 11-mer) peptides eluted from HLA-B*51:01 complexes, and abrogated the predominance of Leucine at P1. Interestingly knockdown of ERAP1 altered the length and N-terminal residue usage of non-Ala2&Pro2 and Ala2 but not the Pro2 peptides. Finally, ERAP1 silencing regulated the expression levels of cell surface HLA-B*51 in a cell type - dependent manner. The HLA-B*51:01 peptidome includes a surprisingly high proportion of unconventional non-Pro/Ala2 peptides which are increased by ERAP1 silencing, mimicking the loss-of-function BD risk variant.

Dataset Information

Identification of HLA-A11:01 and A02:01-restricted EBV Pep-tides using HLA Peptidomics

Dataset's files

Publications

Identification of HLA-A11:01 and A02:01-Restricted EBV Peptides Using HLA Peptidomics.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Identification of HLA-A*11:01 and A*02:01-restricted EBV Pep-tides using HLA Peptidomics

Dataset's files

Publications

Identification of HLA-A*11:01 and A*02:01-Restricted EBV Peptides Using HLA Peptidomics.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Identification of HLA-A11:01 and A02:01-restricted EBV Pep-tides using HLA Peptidomics

Identification of HLA-A11:01 and A02:01-Restricted EBV Peptides Using HLA Peptidomics.