Dataset Information

Proteome of the Developing Murine Lens Through Mass Spectrometry

ABSTRACT: The proteome is a term used for the entire catalog of proteins present in a cell at any time. In here, we investigated the proteome of developing mouse lens through mass spectrometry-based protein sequencing. We extracted mouse lenses at six developmental time points, which included two embryonic (E15 and E18) and four postnatal stages (P0, P3, P6, and P9). The lenses from each time point were preserved in three distinct pools to serve as biological replicates for each developmental stage. The total cellular protein was extracted from the lens, digested with trypsin and labeled with 6-plex isobaric tandem mass tags (TMT) for three independent 6-plex TMT experiments. A total of 6,117 proteins were identified in the mouse ocular lens in at least one of the above-mentioned six developmental time points. Of these, 6,117 proteins were present in one TMT set, 4,325 in two, and 2,972 were present in three TMT sets, respectively. Majority of the proteins exhibit steady expression; however, we identified 162 proteins that exhibited an 8-fold differential (higher or lower) expression during the developmental time course compared to their levels at E15. The lens proteome is comprised of diverse proteins that have distinct biological properties and functional characteristics including a total of 404 proteins that have been associated with autophagy. We have established a comprehensive profile of the developing murine lens proteome. This repository will be helpful in identifying critical components of lens development and processes essential for the maintenance of its transparency.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Lens Of Camera-type Eye, Lens

SUBMITTER: OM Genetics

LAB HEAD: S. Amer Riazuddin

PROVIDER: PXD006381 | Pride | 2018-01-22

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	AR_Lens_TMT_7plex_Set_1_F01.raw	Raw
	AR_Lens_TMT_7plex_Set_1_F02.raw	Raw
	AR_Lens_TMT_7plex_Set_1_F03.raw	Raw
	AR_Lens_TMT_7plex_Set_1_F04.raw	Raw
	AR_Lens_TMT_7plex_Set_1_F05.raw	Raw

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Publications

Proteome Profiling of Developing Murine Lens Through Mass Spectrometry.

Khan Shahid Y SY Ali Muhammad M Kabir Firoz F Renuse Santosh S Na Chan Hyun CH Talbot C Conover CC Hackett Sean F SF Riazuddin S Amer SA

Investigative ophthalmology & visual science 20180101 1

<h4>Purpose</h4>We previously completed a comprehensive profile of the mouse lens transcriptome. Here, we investigate the proteome of the mouse lens through mass spectrometry-based protein sequencing at the same embryonic and postnatal time points.<h4>Methods</h4>We extracted mouse lenses at embryonic day 15 (E15) and 18 (E18) and postnatal day 0 (P0), 3 (P3), 6 (P6), and 9 (P9). The lenses from each time point were preserved in three distinct pools to serve as biological replicates for each dev ...[more]

PMID: 29332127

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