Proteomics

Dataset Information

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OVA N-glycans PGC-LC-MSMS - Large-scale identification and visualization of N-glycans with primary structures using GlySeeker


ABSTRACT: With PNGase F digestion, PGC enrichment,OVA N-glycans were analyzed using PGC nanoLC-ESI-MS/MS.Datasets were analyzed by the N-glycan database search engine called GlycanGoggle developed in our group.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Gallus Gallus (chicken)

SUBMITTER: Kaijie Xiao  

LAB HEAD: Zhixin Tian

PROVIDER: PXD006400 | Pride | 2019-01-17

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
OVA_N-glycans_PGC-LC-MSMS_neg.m_bin Other
OVA_N-glycans_PGC-LC-MSMS_neg.raw Raw
OVA_N-glycans_PGC-LC-MSMS_neg.txt Txt
OVA_N-glycans_PGC-LC-MSMS_pos.m_bin Other
OVA_N-glycans_PGC-LC-MSMS_pos.raw Raw
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Publications

Large-scale identification and visualization of N-glycans with primary structures using GlySeeker.

Xiao Kaijie K   Wang Yue Y   Shen Yun Y   Han Yuyin Y   Tian Zhixin Z  

Rapid communications in mass spectrometry : RCM 20180101 2


<h4>Rationale</h4>Most of the current popular tandem mass spectrometers have the capability of resolving the primary structures (monosaccharide composition, sequence and linkage) of N-glycans; however, compositions or putative structures have mostly been reported so far. Identification and visualization tools of N-glycans are needed.<h4>Methods</h4>The isotopic mass-to-charge ratio and envelope fingerprinting algorithm, which has been successfully used for intact protein database search and iden  ...[more]

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