Proteomics

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Quantitative proteomic characterization of lung-MSC and bone marrow-MSC using DIA-mass spectrometry


ABSTRACT: Mesenchymal stromal cells (MSC) are ideal candidates for cell therapies, due to their immune-regulatory and regenerative properties. We have previously reported that lung-derived MSC are tissue-resident cells with lung-specific properties compared to bone marrow-derived MSC. Assessing relevant molecular differences between lung-MSC and bone marrow-MSC is important, given that such differences may impact their behavior and potential therapeutic use. Here, we present an in-depth mass spectrometry (MS) based strategy to investigate the proteomes of lung-MSC and bone marrow-MSC. The MS-strategy relies on label free quantitative data-independent acquisition (DIA) analysis and targeted data analysis using a MSC specific spectral library. We identified several significantly differentially expressed proteins between lung-MSC and bone marrow-MSC within the cell layer (352 proteins) and in the conditioned medium (49 proteins). Bioinformatics analysis revealed differences in regulation of cell proliferation, which was functionally confirmed by decreasing proliferation rate through Cytochrome P450 stimulation. Our study reveals important tissue-specific differences within proteome and matrisome profiles between lung- and bone marrow-derived MSC that may influence their behavior and affect the clinical outcome when used for cell-therapy.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Lung, Stem Cell, Cell Culture, Bone Marrow

SUBMITTER: Sara Rolandsson Enes  

LAB HEAD: Gunilla Westergren-Thorsson

PROVIDER: PXD006419 | Pride | 2018-10-18

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Emma_H1505_004.pep.xml Pepxml
Emma_H1505_004.raw Raw
Emma_H1505_005.pep.xml Pepxml
Emma_H1505_005.raw Raw
Emma_H1505_006.pep.xml Pepxml
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Publications

Quantitative proteomic characterization of lung-MSC and bone marrow-MSC using DIA-mass spectrometry.

Rolandsson Enes Sara S   Åhrman Emma E   Palani Anitha A   Hallgren Oskar O   Bjermer Leif L   Malmström Anders A   Scheding Stefan S   Malmström Johan J   Westergren-Thorsson Gunilla G  

Scientific reports 20170824 1


Mesenchymal stromal cells (MSC) are ideal candidates for cell therapies, due to their immune-regulatory and regenerative properties. We have previously reported that lung-derived MSC are tissue-resident cells with lung-specific properties compared to bone marrow-derived MSC. Assessing relevant molecular differences between lung-MSC and bone marrow-MSC is important, given that such differences may impact their behavior and potential therapeutic use. Here, we present an in-depth mass spectrometry  ...[more]

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