Unknown,Transcriptomics,Genomics,Proteomics

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Nuclear receptors Nur77 and Nurr1 modulate mesenchymal stromal cell migration

ABSTRACT: Expression analysis of migrating and non-migrating mesenchymal stromal cells (MSC) in fetal bone marrow Keywords: fetal bone marrow, mesenchymal stromal cells, migration, gene expression, genomics Three biological replates for both migrating and non-migrating mesenchymal stromal cells (MSC) in fetal bone marrow

ORGANISM(S): Homo sapiens

SUBMITTER: Marijke Maijenburg

PROVIDER: E-GEOD-24892 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Nuclear receptors Nur77 and Nurr1 modulate mesenchymal stromal cell migration.

Maijenburg Marijke W MW Gilissen Christian C Melief Sara M SM Kleijer Marion M Weijer Kees K Ten Brinke Anja A Roelofs Helene H Van Tiel Claudia M CM Veltman Joris A JA de Vries Carlie J M CJ van der Schoot C Ellen CE Voermans Carlijn C

Stem cells and development 20110601 2

Detailed understanding of mesenchymal stromal cells (MSC) migration is imperative for future cellular therapies. To identify genes involved in the process of MSC migration, we generated gene expression profiles of migrating and nonmigrating fetal bone marrow MSC (FBMSC). Only 12 genes showed differential expression in migrating versus nonmigrating FBMSC. The nuclear receptors Nur77 and Nurr1 showed the highest expression in migratory MSC. Nur77 and Nurr1 are members of NR4A nuclear orphan recept ...[more]

PMID: 21480782

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Project description:Background: Mesenchymal stromal cells (MSC) have been proposed as a future cell based therapy for lung diseases like bronchiolitis obliterans syndrome (BOS). Theoretically, it might be beneficial to use lung resident MSC already adapted to the pulmonary environment. We therefore aimed to compare lung MSC with the well-characterized bone marrow MSC. Furthermore, MSC isolated from lung-transplanted patients with BOS were compared to MSC isolated from good outcome recipients. Methods: MSC were isolated from bone marrow (BM) and adult/fetal lung tissues and compared by a comprehensive panel of assays. Results:The gene expression profiles of adult lung derived and fetal lung derived MSC were very similar compared to BM derived MSC, with only 235 and 338 genes, respectively, being significantly differently expressed. Out of the 235 genes that were significantly different between adult lung derived and BM derived MSC, 90 genes were higher expressed in the lung derived MSC. In case of fetal lung derived MSC, 166 genes were higher expressed compared to the BM derived MSC. Interestingly, only, 89 genes were found to be expressed differently when comparing BM derived MSC to both, fetal and adult lung derived MSC, indicating that these genes are lung specific. Next, we went on to evaluate if MSC isolated from biopsies of lung-transplanted patients with BOS differed compared to patients without BOS, i.e. good outcome recipients. Here we found that four genes (Sox9, FAR2, LOC728855 and NDUFS5) were significantly higher expressed in MSC isolated from BOS patients Conclusions: This data demonstrates that lung resident MSC possess lung specific properties that should be taken into considerations when using MSC for cell-based therapy in severe lung disorders like BOS. These results also show that MSC isolated from lung-transplanted patients with BOS do not have an altered phenotype. Comparison of gene expression of MSC isolated from bone marrow, adult lung and fetal lung.

Dataset Information

Nuclear receptors Nur77 and Nurr1 modulate mesenchymal stromal cell migration

Publications

Nuclear receptors Nur77 and Nurr1 modulate mesenchymal stromal cell migration.

Similar Datasets

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