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Distinct proteome remodeling of industrial Saccharomyces cerevisiae in response to prolonged thermal stress or transient heat shock

ABSTRACT: To gain deep understanding of yeast cell response to heat stress, multiple laboratory strains have been intensively studied by genome-wide expression analysis for mechanistic dissection of classical heat shock response. However, robust industrial strains of S. cerevisiae have hardly been explored in global analysis for elucidating the mechanism of thermotolerant response (TR) during fermentation. Herein, we employed DIA/SWATH–based proteomic workflows to characterize proteome remodeling of an industrial strain ScY01 responding to prolonged thermal stress or transient heat shock.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Xiao Weidi

LAB HEAD: Wenqing Shui

PROVIDER: PXD006464 | Pride | 2020-11-05

REPOSITORIES: Pride

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			Action	DRS
	20151024_T0_2sw33exp_1.wiff	Wiff
	20151024_T0_2sw33exp_1.wiff.scan	Wiff
	20151024_T0_2sw33exp_2.wiff	Wiff
	20151024_T0_2sw33exp_2.wiff.scan	Wiff
	20151024_T0_3sw33exp_1.wiff	Wiff

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Publications

Identification of osteoporosis markers through bioinformatic functional analysis of serum proteome.

Lv Mengying M Cui Chuanlong C Chen Peng P Li Ziqi Z

Medicine 20200901 39

Osteoporosis is a severe chronic skeletal disorder that increases the risks of disability and mortality; however, the mechanism of this disease and the protein markers for prognosis of osteoporosis have not been well characterized. This study aims to characterize the imbalanced serum proteostasis, the disturbed pathways, and potential serum markers in osteoporosis by using a set of bioinformatic analyses. In the present study, the large-scale proteomics datasets (PXD006464) were adopted from the ...[more]

PMID: 32991410

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2011-11-09 | E-GEOD-24197 | biostudies-arrayexpress

Systemic Comparison of Heat Shock Response Induced by Heat Shock and a Proteasome Inhibitor in Mouse Fibrosarcoma Cells and Their Thermotolerant Counterparts

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Project description:Furfural, phenol and acetic acid, generated during cellulosic material pretreatment, are the representative inhibitors to yeast used for ethanol production. The responses to these inhibitors in industrial yeast and the corresponding adapted strains were analyzed. Experiment Overall Design: We analyzed the transient response to inhibitors and the different transcriptions in industrial yeast and furfural-, phenol-, and acetic acid-adapted strains. Industrial yeast and the adapted strains were collected at 20 minutes after inhibitor addition. The reference samples for industrial yeast and adapted strains were collected at the same time without inhibitor addition. 2 replicates for each strain/treatment were analyzed.

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