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Quantitative Proteomic Atlas Uncovering the widespread Ubiquitylation Regulation in the Embryo of Germinating Rice Seed

ABSTRACT: Using high affinity K-Ɛ-GG antibody integrating highly sensitive MS, we investigated the quantitative atlas of ubiquitylome as well as the proteome in the embryos of germinated rice seed. A list of 2576 lysine ubiquitinated sites in 1171 proteins,mainly involved in protein process, nucleic acid stability, hormonal regulation, and metabolism,were identified in the first 0, 12 and 24 hours after seed imbibition (HAI). The additional ubiquitin were more likely to attacked the lysine sites that located in the polar acidic other than basophilic regions. Of these modified proteins,1435 sites in 781 proteins were significantly changed in abundance (folds change> 2, P<0.05), most ubiquitinated sites were increased rapidly in the first 12 HAI.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Oryza Sativa (rice)

TISSUE(S): Embryo, Root

SUBMITTER: Dongli He

LAB HEAD: Pingfang Yang

PROVIDER: PXD006525 | Pride | 2022-02-23

REPOSITORIES: pride

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			Action	DRS
	1-0h_rep1.raw	Raw
	1-0h_rep2.raw	Raw
	1-0h_rep3.raw	Raw
	1-12h_rep1.raw	Raw
	1-12h_rep2.raw	Raw

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