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Characterization of seminal vesicle proteome of cactus field mouse (Peromyscus eremicus) in hydrated and dehydrated conditions

ABSTRACT: ABSTRACT: The cactus mouse (Peromyscus eremicus) is a desert-specialized rodent that experiences both chronic and acute dehydration in the Southwestern United States. Our previous research has generated substantial transcriptomic data on P. eremicus kidneys, testes, epididymis, and vas deferens in individuals exposed to hydrated and dehydrated conditions; however, the study described here is the first to describe a seminal vesicle proteome for this species. We have produced a seminal vesicle proteome from P. eremicus with free access to water and mice that were acutely dehydrated to generate a dataset that is comprehensive for both alternative water-availability states experienced by this species. We have also provided gene ontologies for this proteome using PANTHER. This proteome will provide a crucial resource for future studies characterizing the genetic and proteomic responses of reproductive tissues to drought in this rodent. Furthermore, an enhanced understanding of survival and reproductive responses (and adaptations) to dehydration is particularly relevant to clinical work aiming to minimize adverse human impacts as climate change continues to increase the incidence of drought.

INSTRUMENT(S): LTQ Orbitrap, LTQ XL

ORGANISM(S): Peromyscus Eremicus

TISSUE(S): Seminal Vesicle Fluid

SUBMITTER: Donna Hogan

LAB HEAD: Dr. Matt MacManes

PROVIDER: PXD006532 | Pride | 2022-03-02

REPOSITORIES: Pride

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Project description:BACKGROUND: Prostate cancer is the most frequently diagnosed cancer among men in the United States. In contrast, cancer of the seminal vesicle is exceedingly rare, despite that the prostate and seminal vesicle share similar histology, secretory function, androgen dependency, blood supply, and (in part) embryonic origin. We hypothesized that gene-expression differences between prostate and seminal vesicle might inform mechanisms underlying the higher incidence of prostate cancer. METHODS: Whole-genome DNA microarrays were used to profile gene expression of 11 normal prostate and 7 seminal vesicle specimens (including 6 matched pairs) obtained from radical prostatectomy. Supervised analysis was used to identify genes differentially expressed between normal prostate and seminal vesicle, and this list was then cross-referenced to genes differentially expressed between normal and cancerous prostate. Expression patterns of selected genes were confirmed by immunohistochemistry using a tissue microarray. We identified 32 genes that displayed a highly statistically-significant expression pattern with highest levels in seminal vesicle, lower levels in normal prostate, and lowest levels in prostate cancer. Among these genes was the known candidate prostate tumor suppressor GSTP1 (involved in xenobiotic detoxification). The expression pattern of GSTP1 and four other genes, ABCG2 (xenobiotic transport), CRABP2 (retinoic acid signaling), GATA3 (lineage-specific transcription) and SLPI (immune response), was confirmed by immunohistochemistry. CONCLUSIONS: Our findings identify candidate prostate cancer genes whose reduced expression in prostate (compared to seminal vesicle) may be permissive to prostate cancer initiation. Such genes and their pathways may inform mechanisms of prostate carcinogenesis, and suggest new opportunities for prostate cancer prevention. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Disease State: normal prostate vs normal seminal vesicle sample Individual Keywords: Logical Set cDNA microarrays from the Stanford Functional Genomics Facility were used for expression profiling of 11 normal prostate and 7 seminal vesicle specimens (6 of which were matched pairs), against a universal RNA reference. Extracted expression ratios were normalized by array then mean centered by gene, and expression differences between normal prostate and seminal vesicle identified using Significance Analysis of Microarrays (SAM).

			Action	DRS
	O5071508.RAW	Raw
	O5071508.mgf	Mgf
	O5071508.mzid.gz	Mzid
	O5071508.pride.mgf.gz	Mgf
	O5071508.pride.mztab.gz	Mztab

Dataset Information

Characterization of seminal vesicle proteome of cactus field mouse (Peromyscus eremicus) in hydrated and dehydrated conditions

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