Dataset Information

ChaFRAtip - Simple, scalable and ultra-sensitive tip-based identification of protease substrates

ABSTRACT: Enrichment of N-terminal peptides using HPLC-based ChaFRADIC and the novel tip-based ChaFRAtip method.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: René Zahedi

LAB HEAD: Rene Zahedi

PROVIDER: PXD006595 | Pride | 2018-01-24

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Comparison_HPLC_vs_Tips________HPLC_1FR_rep1.raw	Raw
	Comparison_HPLC_vs_Tips________HPLC_1FR_rep2.raw	Raw
	Comparison_HPLC_vs_Tips________HPLC_1FR_rep3.raw	Raw
	Comparison_HPLC_vs_Tips________HPLC_2FR_rep1.raw	Raw
	Comparison_HPLC_vs_Tips________HPLC_2FR_rep2.raw	Raw

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Publications

Simple, scalable, and ultrasensitive tip-based identification of protease substrates.

Shema Gerta G Nguyen Minh T N MTN Solari Fiorella A FA Loroch Stefan S Venne A Saskia AS Kollipara Laxmikanth L Sickmann Albert A Verhelst Steven H L SHL Zahedi René P RP

Molecular & cellular proteomics : MCP 20180122 4

Proteases are in the center of many diseases, and consequently, proteases and their substrates are important drug targets as represented by an estimated 5-10% of all drugs under development. Mass spectrometry has been an indispensable tool for the discovery of novel protease substrates, particularly through the proteome-scale enrichment of so-called N-terminal peptides representing endogenous protein N termini. Methods such as combined fractional diagonal chromatography (COFRADIC)<sup>1</sup> an ...[more]

PMID: 29358340

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