Polysome profiling and ribosome footprinting of Drosophila mature oocyte and activated egg
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ABSTRACT: We use mRNA-seq in combination with polysome profiling to determine translational status for all mRNAs in Drosophila mature oocytes and activated eggs. Puromycin-treated lysates are used as a negative control in polysome profiling experiments. Additionally, we use ribosome footprinting to globally measure translational efficiency of mRNAs in wild type mature oocytes as well as wild type and png mutant activated eggs. Lysates of hand-dissected Drosophila mature oocytes (containing ~540 M-NM-
ORGANISM(S): Drosophila melanogaster
SUBMITTER: Iva Kronja
PROVIDER: E-GEOD-52799 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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