Dataset Information

Proteomic profiling of ethanol tolerance in yeast

ABSTRACT: For yeast cells, tolerance to high levels of ethanol is vital both in their natural environment and in industrially relevant conditions. We recently genotyped experimentally evolved yeast strains, tolerant to high levels of ethanol. In this study, we elucidate how the genotype and phenotype are related to each other on the molecular level through an integrated analysis of mutation data with protein abundance profiles from six evolved, ethanol-tolerant clones. We constructed a protein network of regulatory proteins, mutated and differentially regulated proteins. This resulted in the identification of a rewired regulatory network after adaptation to high levels of ethanol.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

TISSUE(S): Culture Condition:yeast Extract-grown Cell, Cell Line Cell

SUBMITTER: Impens Francis

LAB HEAD: Kevin Verstrepen

PROVIDER: PXD006631 | Pride | 2020-12-03

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	H00641_77a_YPD_yeast_PJ_testsample.raw	Raw
	H00645_5466_78a_YPD_yeast_PJ.raw	Raw
	H00647_5466_79a_YPD_yeast_PJ.raw	Raw
	H00649_5466_88a_YPD_yeast_PJ.raw	Raw
	H00651_5564_79d_YPD_yeast_PJ.raw	Raw

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Publications

Integrated Multi-Omics Analysis of Mechanisms Underlying Yeast Ethanol Tolerance.

Šoštarić Nikolina N Arslan Ahmed A Carvalho Bernardo B Plech Marcin M Voordeckers Karin K Verstrepen Kevin J KJ van Noort Vera V

Journal of proteome research 20210708 8

For yeast cells, tolerance to high levels of ethanol is vital both in their natural environment and in industrially relevant conditions. We recently genotyped experimentally evolved yeast strains adapted to high levels of ethanol and identified mutations linked to ethanol tolerance. In this study, by integrating genomic sequencing data with quantitative proteomics profiles from six evolved strains (data set identifier PXD006631) and construction of protein interaction networks, we elucidate exac ...[more]

PMID: 34236875

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