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Comparison of the transcriptional response to ethanol in laboratory and wild yeast strains

ABSTRACT: An S288c-derived lab strain of Saccharomyces cerevisiae has lower ethanol resistance than either the vineyard strain M22 or the oak strain YPS163. We performed a 60 minute time-course experiment in the presence of 5% ethanol and compared the transcriptional response to ethanol between strains. We additionally analyzed the time-point of maximal response (30 min.) in wild type cells, YPS163 msn2D, and YPS163 hap1D cells. The genomic expression response to 5% ethanol stress was measured in 3 different strain backgrounds, and 2 different mutant backgrounds. Basal gene expression was also compared in two non-lab strains to the S288c reference. A single replicate was performed for the time-course experiment. All of the single time-point experiments were performed using biological triplicates.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Jeffrey Lewis

PROVIDER: E-GEOD-22904 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Exploiting natural variation in Saccharomyces cerevisiae to identify genes for increased ethanol resistance.

Lewis Jeffrey A JA Elkon Isaac M IM McGee Mick A MA Higbee Alan J AJ Gasch Audrey P AP

Genetics 20100920 4

Ethanol production from lignocellulosic biomass holds promise as an alternative fuel. However, industrial stresses, including ethanol stress, limit microbial fermentation and thus prevent cost competitiveness with fossil fuels. To identify novel engineering targets for increased ethanol tolerance, we took advantage of natural diversity in wild Saccharomyces cerevisiae strains. We previously showed that an S288c-derived lab strain cannot acquire higher ethanol tolerance after a mild ethanol pretr ...[more]

PMID: 20855568

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