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SUMO proteomics in Caenorhabditis elegans

ABSTRACT: Comprehensive list of SUMO targets from the nematode Caenorhabditis elegans. SUMO conjugates isolated from transgenic worms carrying 8His and GFP tagged SUMO. The constructs rescues the lethal knock-out of a single SUMO gene, smo-1. SUMO conjugates where isolated from heat shock, arsenite exposure, and UV treated SUMO-GFP worms as well as from control non treated animals. In parallel identical purification procedure was performed with non-transgenic worms and proteins identified with this control where excluded.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Caenorhabditis Elegans

SUBMITTER: Krzysztof Drabikowski

LAB HEAD: Michał Dadlez

PROVIDER: PXD006644 | Pride | 2018-01-24

REPOSITORIES: Pride

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			Action	DRS
	31203krzys_SUMO_SDS-.raw	Raw
	31203krzys_SUMO_SDS.raw	Raw
	40731krzysioD_S1_dmso.raw	Raw
	40731krzysioD_S2_dmso.raw	Raw
	40813krzys_N2_1.raw	Raw

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Publications

Comprehensive list of SUMO targets in Caenorhabditis elegans and its implication for evolutionary conservation of SUMO signaling.

Drabikowski Krzysztof K Ferralli Jacqueline J Kistowski Michal M Oledzki Jacek J Dadlez Michal M Chiquet-Ehrismann Ruth R

Scientific reports 20180118 1

Post-translational modification by small ubiquitin-related modifier (SUMO) is a key regulator of cell physiology, modulating protein-protein and protein-DNA interactions. Recently, SUMO modifications were postulated to be involved in response to various stress stimuli. We aimed to identify the near complete set of proteins modified by SUMO and the dynamics of the modification in stress conditions in the higher eukaryote, Caenorhabditis elegans. We identified 874 proteins modified by SUMO in the ...[more]

PMID: 29348603

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