Dataset Information

HEp-2 TcdB - Glucosyltransferase dependent and independent effects of TcdB on the proteome of HEp-2 cells

ABSTRACT: Human epithelial cell line HEp-2 was treated with Clostridium difficle toxin A. SILAC labeled samples were analyzed by shotgun LC-MS.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

DISEASE(S): Clostridium Difficile Colitis

SUBMITTER: Johannes Junemann

LAB HEAD: Andreas Pich

PROVIDER: PXD006658 | Pride | 2017-06-20

REPOSITORIES: Pride

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Publications

Glucosyltransferase-dependent and -independent effects of TcdB on the proteome of HEp-2 cells.

Erdmann Jelena J Junemann Johannes J Schröder Anke A Just Ingo I Gerhard Ralf R Pich Andreas A

Proteomics 20170718 15-16

Toxin B (TcdB) of the nosocomial pathogen C. difficile has been reported to exhibit a glucosyltransferase-dependent and -independent effect on treated HEp-2 cells at toxin concentration above 0.3 nM. In order to investigate and further characterize both effects epithelial cells were treated with wild type TcdB and glucosyltransferase-deficient TcdB<sub>NXN</sub> and their proteomes were analyzed by LC-MS. Triplex SILAC labeling was used for quantification. Identification of 5212 and quantificati ...[more]

PMID: 28612519

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