Proteomics

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Human antibodies enhance association of macrophages to Candida albicans and reduce fungal-mediated epithelial damage

ABSTRACT: Candida albicans is a common commensal on human mucosal surfaces, but can become pathogenic, e.g. if the host is immunocompromised. While neutrophils, macrophages and T cells-driven activation of neutrophils are regarded as major players in the defence against C. albicans, the role of B cells and the protective function of their antibodies are less well characterized. In this study, we show that human serum antibodies are able to enhance the the association of human macrophages with C. albicans cells . Human serum antibodies are also capable of reducing the growth of the fungi as well as inhibiting adhesion to epithelial cells. Furthermore, human serum antibodies impair C. albicans’ invasion into human oral epithelial cells by blocking induced endocytosis and consequently host cell damage. While aspartic proteases secreted by C. albicans were able to cleave human IgG, this process does not appear to affect the protective function of human antibodies in adhesion. Thus, humans are equipped with antiC. albicans antibodies, which can enhance antifungal activities and which can prevent fungal mediated epithelial damage (even in immunocompromised settings).

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Serum

SUBMITTER: Thomas Krüger  

LAB HEAD: Axel A. Brakhage

PROVIDER: PXD026082 | Pride | 2022-04-04

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
MW36-7A-Chymotrypsin.fasta Fasta
MW36-7A-Chymotrypsin.mgf Mgf
MW36-7A-Chymotrypsin.msf Msf
MW36-7A-Chymotrypsin.pdResult Other
MW36-7A-Chymotrypsin.pep.xml Pepxml
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Publications

<i>Candida albicans</i> is a common commensal on human mucosal surfaces, but can become pathogenic, e.g. if the host is immunocompromised. While neutrophils, macrophages and T cells are regarded as major players in the defense against pathogenic <i>C. albicans</i>, the role of B cells and the protective function of their antibodies are less well characterized. In this study, we show that human serum antibodies are able to enhance the association of human THP-1 monocyte-like cells with <i>C. albi  ...[more]