Dataset Information

COPD lungs show an attached stratified mucus layer resembling the protective colonic mucus

ABSTRACT: Mucus accumulation is a key feature of respiratory diseases such as cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD). This is associated with goblet cell metaplasia and mucin overexpression, which can be induced by the increased activity of neutrophil elastase. The aim of the study was to characterization of mucus and epithelial cell proteomics in a porcine pancreatic elastase (PPE) mouse model of COPD/CF. The major proteins detected in mucus plugs obtained from PPE-treated mice included mucins Muc5ac and Muc5b, mucus-related proteins Clca1, Fcgbp, and Bpifb1. These proteins were upregulated in bronchoalveolar lavage (BAL) fluid and epithelial cells in mice exposed to elastase. Similar changes were found in BAL fluid of COPD patients.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Lung, Epithelial Cell

DISEASE(S): Chronic Obstructive Pulmonary Disease

SUBMITTER: Liisa Arike

LAB HEAD: Liisa Arike

PROVIDER: PXD006770 | Pride | 2018-10-22

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	160708_QEx_LA_MS571_01_targ.raw	Raw
	160708_QEx_LA_MS571_02_targ.raw	Raw
	160708_QEx_LA_MS571_03_targ.raw	Raw
	160708_QEx_LA_MS571_04_targ.raw	Raw
	160708_QEx_LA_MS571_05_targ.raw	Raw

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Publications

Attached stratified mucus separates bacteria from the epithelial cells in COPD lungs.

Fernández-Blanco Joan Antoni JA Fakih Dalia D Arike Liisa L Rodríguez-Piñeiro Ana M AM Martínez-Abad Beatriz B Skansebo Elin E Jackson Sonya S Root James J Singh Dave D McCrae Christopher C Evans Christopher M CM Åstrand Annika A Ermund Anna A Hansson Gunnar C GC

JCI insight 20180906 17

The respiratory tract is normally kept essentially free of bacteria by cilia-mediated mucus transport, but in chronic obstructive pulmonary disease (COPD) and cystic fibrosis (CF), bacteria and mucus accumulates instead. To address the mechanisms behind the mucus accumulation, the proteome of bronchoalveolar lavages from COPD patients and mucus collected in an elastase-induced mouse model of COPD was analyzed, revealing similarities with each other and with the protein content in colonic mucus. ...[more]

PMID: 30185674

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