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Proteogenomic study of Clavibacter michiganensis subsp. michiganensis strain NCPPB382

ABSTRACT: Clavibacter michiganensis subsp. michiganensis is an important Gram-positive phytopathogenic bacteria that causes bacterial wilt and canker in tomato. The genome of the type strain, NCPPB382, has been sequenced and annotated, however comparative genomics suggests that certain regions are under- or misannotated. In order to improve the genome annotation, we have undertaken a proteogenomic study of this important pathogen. Samples were grown in culture and the proteome of the pellet and supernatant were analyzed separately using shotgun HPLC-MS/MS. These proteomics datasets were analyzed and a number of missing gene were found and a number of existing gene calls were modified.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Clavibacter Michiganensis Subsp. Michiganensis Ncppb 382

SUBMITTER: Paul Stodghill

LAB HEAD: Paul Stodghill

PROVIDER: PXD006787 | Pride | 2019-01-31

REPOSITORIES: pride

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			Action	DRS
	Cmm_382_extended.fasta	Fasta
	cmm382_pellet_1.msf	Msf
	cmm382_pellet_1.pep.xml	Pepxml
	cmm382_pellet_1_F01.raw	Raw
	cmm382_pellet_1_F02.raw	Raw

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Publications

Proteome Profile and Genome Refinement of the Tomato-Pathogenic Bacterium Clavibacter michiganensis subsp. michiganensis.

Peritore-Galve F Christopher FC Schneider David J DJ Yang Yong Y Thannhauser Theodore W TW Smart Christine D CD Stodghill Paul P

Proteomics 20190308 7

PMID: 30648817

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