Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

S.lividans TK24 protease deletions

ABSTRACT: Characterization of S.lividans TK24 secreted proteome changes upon deletion of certain proteases.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Streptomyces Lividans Tk24

SUBMITTER: Konstantinos Tsolis

LAB HEAD: Tassos Economou

PROVIDER: PXD006819 | Pride | 2018-06-28

REPOSITORIES: pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SL000_rep1.raw	Raw
	SL000_rep2.raw	Raw
	SL000_rep3.raw	Raw
	SL000_rep4.raw	Raw
	SL000_rep5.raw	Raw

Items per page:

1 - 5 of 49

Publications

Multi-Omics and Targeted Approaches to Determine the Role of Cellular Proteases in Streptomyces Protein Secretion.

Busche Tobias T Tsolis Konstantinos C KC Koepff Joachim J Rebets Yuriy Y Rückert Christian C Hamed Mohamed B MB Bleidt Arne A Wiechert Wolfgang W Lopatniuk Mariia M Yousra Ahmed A Anné Jozef J Karamanou Spyridoula S Oldiges Marco M Kalinowski Jörn J Luzhetskyy Andriy A Economou Anastassios A

Frontiers in microbiology 20180604

Gram-positive Streptomyces bacteria are profuse secretors of polypeptides using complex, yet unknown mechanisms. Many of their secretory proteins are proteases that play important roles in the acquisition of amino acids from the environment. Other proteases regulate cellular proteostasis. To begin dissecting the possible role of proteases in Streptomyces secretion, we applied a multi-omics approach. We probed the role of the 190 proteases of Streptomyces lividans strain TK24 ...[more]

PMID: 29915569

Similar Datasets

S.lividans TK24 sigma-factor deletions

Project description:Characterization of S.lividans TK24 secreted proteome changes upon deletion of certain sigma factors.

2019-02-16 | PXD006818 | Pride

Profiling protease and protease inhibitor gene expression in tumors and their microenvironment

2008-07-21 | GSE6413 | GEO

Protease Treatment

Project description:Determine if transcripts are released by protease treatment During total RNA isolation cell free lysates from stationary-phase cultures or exponential cultures were treated with one of three proteases or buffer alone. All experimental samples are over a common reference. There are two replicates for each sample.

2010-06-30 | E-GEOD-3686 | biostudies-arrayexpress

Small RNA sequencing study of the nematode phylum

Project description:Comparison of small RNA profiles across nematode evolution Sequencing of small RNAs (15-33nt)

2015-02-01 | E-GEOD-56651 | biostudies-arrayexpress

Polytopic protease downregulation effect on the brain (rogae-affy-arabi-492460)

Project description:Regulation of AD-related genes. Presenilins are intramembrane polytopic proteases. These proteases are critical proteins in pathogenesis of Alzheimer's disease. The function of other polytopic proteases expressed in brains is unknown. Proteins that may potentially interact with PS pathway are of interest to elucidate. In this project we will determine gene expression patterns in the brains of mice with altered expression of polytopic protease. We hypothesize that family of polytopic proteases expressed in brain may potentially interact with presenilin pathway. We will examine 4 RNA samples isolated from brains of mice with significant downregulation of polytopic protease and control animals. Total RNA was extracted, purified according to the manufacturer's protocol and stored at -80C. Keywords: polytopic protease, brain

2008-04-12 | GSE11148 | GEO

Profiling protease and protease inhibitor gene expression in tumors and their microenvironment

Project description:To assist in the identification of proteases, their endogenous inhibitors, and proteins that interact with proteases or proteolytic pathways in biological tissues, a dual species oligonucleotide microarray has been developed in conjunction with Affymetrix, Inc. To investigate the performance of the array, gene expression profiles were analyzed in pure mouse and human samples and orthotopically implanted xenografts of human A549 lung and MDA-MB-231 breast carcinomas. The Hu/Mu ProtIn array is a valuable discovery tool for the identification of components of human and murine proteolytic pathways in health and disease, and has particular utility in the determination of cellular origins of proteases and protease inhibitors in xenograft models of human cancer. Keywords: gene expression profiles were analyzed in pure mouse and human samples and orthotopically implanted xenografts of human A549 lung and MDA-MB-231 breast carcinomas Commercial pan human and mouse total RNA replicate samples (n=3) were profiled. In addition, human ductal stage II or III breast carcinoma biopsies (n=18) were compared to normal human breast biopsies (n=13). Spontaneous spontaneous murine mammary carcinomas from MMTV-PyMT+(FVB/n) (n=8) and MMTV-PyMT+/uPARAP-/-(FVB/n) (n=2) transgenic mice were compared to normal mouse mammary fat pads (n=4; female Rag-1 tm1Mom retired breeders at >6 weeks post lactation). In vitro cultures of MDA-MB-231 human breast carcinoma cells (n=3) and MDA-MB-231 orthotopic xenografts (n=3) were profiled. The xenografts were compared to normal mammary fat pads. A549 human lung adenocarcinoma cells (n=2), A549 orthotopic xenografts (n=3) and normal mouse lung tissue (n = 3) were profiled.

2008-07-20 | E-GEOD-6413 | biostudies-arrayexpress

Lon Protease Promotes Survival of Escherichia coli During Anaerobic Glucose Starvation

Project description:In Escherichia coli, Lon is an ATP-dependent protease which degrades misfolded proteins and certain rapidly-degraded regulatory proteins. Given that oxidatively damaged proteins are generally degraded rather than repaired, we anticipated that Lon deficient cells would exhibit decreased viability during aerobic, but not anaerobic, carbon starvation. We found that the opposite actually occurs. Wild-type and Lon deficient cells survived equally well under aerobic conditions, but Lon deficient cells died more rapidly than the wild-type under anaerobiosis. Microarray analysis revealed that genes of the Clp family of ATP-dependent proteases were induced during aerobic growth but not during anaerobic growth. Thus, Clp may compensate for loss of Lon when cells are in an oxygen containing atmosphere. Under anaerobic carbon starvation conditions, Lon must be active to support survival. Keywords: Other

2007-11-01 | GSE6609 | GEO

Modulation of protease expression by PITX transcription factors regulates protein quality control during aging

Project description:Protein quality control is important for healthy aging and is dysregulated in several age-related diseases. The autophagy-lysosome and ubiquitin-proteasome systems are key for proteostasis but it remains largely unknown whether other proteolytic systems maintain protein quality control during aging. Here, we find that the expression of proteolytic enzymes (proteases/peptidases) distinct from the autophagy-lysosome and ubiquitin-proteasome systems declines during skeletal muscle aging in Drosophila. Age-dependent downregulation of proteases undermines proteostasis, as demonstrated by the increase in detergent-insoluble poly-ubiquitinated proteins and pathogenic huntingtin-polyQ in response to protease RNAi. Transcriptomic analysis identifies Ptx1, homologous to human PITX1/2/3, as a transcriptional regulator of proteases. Specifically, RNAi for Ptx1 increases the expression of age-downregulated proteases and improves protein quality. Moreover, muscle-specific expression of Ptx1 RNAi extends lifespan. These findings indicate that protein quality control is ensured during aging by autophagy/proteasome-independent proteases that degrade misfolded and aggregation-prone proteins and by their transcriptional modulator Ptx1.

2022-08-15 | GSE156346 | GEO

Transgenerational antiviral RNAi does not occur in Orsay virus infection of C. elegans

Project description:Comparison of small RNAs between virus-infected animals and their progeny Sequencing of small RNAs (15-30nt)

2015-01-01 | E-GEOD-60020 | biostudies-arrayexpress

Small RNA sequencing study of C elegans piRNA biogenesis factors

Project description:Identification of defects in piRNAs and piRNA precursors Sequencing of small RNAs (18-30nt). Sample treatments: Secondary siRNAs in C. elegans carry a 5' triphosphate and this has to be removed prior to cloning, hence the use of 5' polyphosphatase treatment to reveal this population. TAP (tobacco acid pyrophosphatase) is another enzyme that removes 5' triphosphate but it also removes 5' CAP structures- we used this to look for piRNA precursor sequences in C. elegans that have a 5' cap.

2014-04-01 | E-GEOD-49220 | biostudies-arrayexpress