Dataset Information

Comparative proteomics of Desulfotomaculum kuznetsovii grown on methanol and ethanol

ABSTRACT: The aim of this study was to unravel the methanol metabolism of Desulfotomaculum kuznetsovii. Anaerobic methylotrophs, such as methanogens and acetogens, use a pathway initiated by a cobalamine-containing methanol methyltransferase, whereas aerobic methylotrophs generally oxidize methanol to formaldehyde through a pathway initiated by a methanol dehydrogenase. Sulfate-respiring cells grown with methanol in the presence and absence of cobalt and vitamin B12 were analyzed and compared with cells grown with lactate or ethanol. Proteome analysis showed the presence of two methanol degrading pathways in D. kuznetsovii: a cobalt-dependent methanol methyltransferase and a cobalt-independent alcohol dehydrogenase. This is the first time that two methanol pathways have been shown to be present in a single microorganism, and we hypothesize this can give D. kuznetsovii a competitive advantage.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Desulfotomaculum Kuznetsovii Dsm 6115

SUBMITTER: Sjef Boeren

LAB HEAD: Sjef Boeren

PROVIDER: PXD006899 | Pride | 2018-01-22

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	DMS100_MeOH5_Rep3_d.raw	Raw
	DMS101_EtOH20Co_Rep1_a.raw	Raw
	DMS102_EtOH20Co_Rep1_b.raw	Raw
	DMS103_EtOH20Co_Rep1_c.raw	Raw
	DMS104_EtOH20Co_Rep1_d.raw	Raw

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Publications

The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways.

Sousa Diana Z DZ Visser Michael M van Gelder Antonie H AH Boeren Sjef S Pieterse Mervin M MM Pinkse Martijn W H MWH Verhaert Peter D E M PDEM Vogt Carsten C Franke Steffi S Kümmel Steffen S Stams Alfons J M AJM

Nature communications 20180116 1

Methanol is generally metabolized through a pathway initiated by a cobalamine-containing methanol methyltransferase by anaerobic methylotrophs (such as methanogens and acetogens), or through oxidation to formaldehyde using a methanol dehydrogenase by aerobes. Methanol is an important substrate in deep-subsurface environments, where thermophilic sulfate-reducing bacteria of the genus Desulfotomaculum have key roles. Here, we study the methanol metabolism of Desulfotomaculum kuznetsovii strain 17< ...[more]

PMID: 29339722

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