Proteomics

Dataset Information

0

Human Mitochondrial NFS1-FXN XL LS-MS/MS


ABSTRACT: Frataxin (FXN) is involved in mitochondrial iron-sulfur (Fe-S) cluster biogenesis and serves to accelerate Fe-S cluster formation. FXN deficiency is associated with Friedreich ataxia, a neurodegenerative disease. Using chemical cross-linking (XL) accompanied with LC-MS/MS, we studied the the interaction between the core complexes Acp-ISD11-NFS1 (AIN) and AIN-ISCU (AINU) with FXN.

INSTRUMENT(S): 6340 Ion Trap LC/MS

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Cell

DISEASE(S): Friedreich Ataxia

SUBMITTER: Kai Cai  

LAB HEAD: John L. Markley

PROVIDER: PXD006928 | Pride | 2018-07-11

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
1_NFS1-Fxn_Markley (F016504).mzid_1_NFS1-Fxn_Markley_(F016504).pride.mgf.gz Mzid
1_NFS1-Fxn_Markley.mztab.gz Mztab
1_NFS1-Fxn_Markley.pride.mztab.gz Mztab
1_NFS1-Fxn_Markley.raw Raw
1_NFS1-Fxn_Markley.zhrs Other
Items per page:
1 - 5 of 7
altmetric image

Publications

Architectural Features of Human Mitochondrial Cysteine Desulfurase Complexes from Crosslinking Mass Spectrometry and Small-Angle X-Ray Scattering.

Cai Kai K   Frederick Ronnie O RO   Dashti Hesam H   Markley John L JL  

Structure (London, England : 1993) 20180705 8


Cysteine desulfurase plays a central role in mitochondrial iron-sulfur cluster biogenesis by generating sulfur through the conversion of L-cysteine to L-alanine and by serving as the platform for assembling other components of the biosynthetic machinery, including ISCU, frataxin, and ferredoxin. The human mitochondrial cysteine desulfurase complex consists of two copies each of NFS1, ISD11, and acyl carrier protein. We describe results from chemical crosslinking coupled with tandem mass spectrom  ...[more]

Similar Datasets

2018-07-11 | PXD006938 | Pride
2018-07-11 | PXD009079 | Pride
2022-03-25 | PXD009128 | Pride
2013-09-23 | E-GEOD-48574 | biostudies-arrayexpress
2013-09-23 | GSE48574 | GEO
2022-04-04 | PXD029519 | Pride
2023-12-27 | PXD045443 | Pride
2023-12-18 | GSE242192 | GEO
2017-04-26 | PXD004920 | Pride
2019-10-07 | PXD010884 | Pride