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Picky - a simple online method designer for targeted proteomics

ABSTRACT: Picky is an online method designer for targeted proteomics. To assess the performance of PRM methods designed by Picky we carried out a benchmark experiment. As reference samples we used different amounts of human proteins spiked into 1.4 µg yeast lysate. These sample where analyzed in PRM and DDA mode. All methods inlcuded a shotgun method (top10 for DDA and top3 for PRM) and can therefore all be analyzed by MaxQuant.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human) Saccharomyces Cerevisiae (baker's Yeast)

TISSUE(S): Cell Culture

SUBMITTER: Henrik Zauber

LAB HEAD: Matthias Selbach

PROVIDER: PXD007039 | Pride | 2017-08-04

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Gladys_20170516_HZ_HS_Yeast_UPS2_DDA.raw	Raw
	Gladys_20170516_HZ_HS_Yeast_UPS2_PRM_F2.raw	Raw
	Gladys_20170516_HZ_HS_Yeast_UPS2_PRM_T.raw	Raw
	Gladys_20170516_HZ_HS_Yeast_UPS3_DDA.raw	Raw
	Gladys_20170516_HZ_HS_Yeast_UPS3_PRM_F.raw	Raw

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Publications

Picky: a simple online PRM and SRM method designer for targeted proteomics.

Zauber Henrik H Kirchner Marieluise M Selbach Matthias M

Nature methods 20180201 3

PMID: 29489744

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