Dataset Information

Proteome analyses define marked differences between N. caninum isolates throughout the tachyzoite lytic cycle

ABSTRACT: Neospora caninum has been recognised as the main cause of transmissible abortion in cattle worldwide. Although the pathogenesis of this disease remains largely unknown, different factors associated to both the host and the parasite have influence in the dynamics of the disease. Intraspecific variation in virulence have been widely shown among N. caninum isolates. However, the molecular basis that govern such variability and the outcome of the infection have not been well-established yet. This study aims to improve the knowledge on the factors governing intra-specific variation in virulence in N. caninum. Quantitative label free LC-MS/MS was used to investigate proteome differences between the high virulent isolate Nc-Spain7 and the low virulent isolate Nc-Spain1H through tachyzoite lytic cycle: at the end of invasion (12 hours), during exponential growth (36 hours) and at early egress (56 hours). The results showed the higher differences in abundance of proteins at invasion and egress, 77 and 62 proteins, respectively. At 36 h, during active parasite replication only 19 proteins were found differentially abundant between isolates. Microneme protein repertoire, involved in parasite invasion and egress, was more abundant in Nc-Spain1H isolate, which displays a lower invasion rate. Soluble effectors related to Toxoplasma gondii virulence (ROP and GRA), proteins of carbohydrate and fatty acid metabolism and stress responses also showed different abundance between isolates. Differential expression analysis by RNAseq technology during tachyzoite egress from the host-cell (56 hours) was also performed showing an expression profile of genes associated with the bradyzoite stage the low virulent and low growing NcSpain1H isolate. The differences in proteome and RNA expression profiles between these two isolates reveal interesting insights into likely mechanisms involved in specific phenotypic traits and virulence in N. caninum.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Primates Neospora Caninum (coccidian Parasite)

SUBMITTER: Dong Xia

LAB HEAD: Jonathan Wastling

PROVIDER: PXD007062 | Pride | 2017-11-17

REPOSITORIES: pride

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Integrative transcriptome and proteome analyses define marked differences between Neospora caninum isolates throughout the tachyzoite lytic cycle.

Horcajo P P Xia D D Randle N N Collantes-Fernández E E Wastling J J Ortega-Mora L M LM Regidor-Cerrillo J J

Journal of proteomics 20171114

Neospora caninum is one of the main causes of transmissible abortion in cattle. Intraspecific variations in virulence have been widely shown among N. caninum isolates. However, the molecular basis governing such variability have not been elucidated to date. In this study label free LC-MS/MS was used to investigate proteome differences between the high virulence isolate Nc-Spain7 and the low virulence isolate Nc-Spain1H throughout the tachyzoite lytic cycle. The results showed greater differences ...[more]

PMID: 29154927

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