Dataset Information

Proteomic characterisation of host-pathogen interactions during bovine trophoblast cell line infection by Neospora caninum

ABSTRACT: Despite the importance of bovine neosporosis, important knowledge gaps remain concerning pathogenic mechanisms of N. caninum. The infection of the placenta is a crucial event in the pathogenesis of the disease, however, very little is known about the relation of the parasite with this target organ. Recent studies have shown that isolates with important variations in virulence also show different interactions with the bovine trophoblast cell line F3, involving proliferative capacity and transcriptome host cell modulation. Herein, we use the same model of infection to study the interaction of Neospora with these target cells at the proteomic level using LC-MS/MS over the course of the parasite lytic cycle. We also analyzed the proteome differences between high (Nc-Spain7)- and low (Nc-Spain1H)- virulence isolates. The results showed mitochondrial processes and metabolism as the main point of Neospora-host interactions. Interestingly, Nc-Spain1H infection showed the highest level of influence on host cell proteome in contrast to Nc-Spain7 infection. These results confirm Nc-Spain7 capacities to evade host cell response and establish infection more effectively.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Neospora Caninum (strain Liverpool)

TISSUE(S): Trophoblast Cell, Trophoblast Cell Line

SUBMITTER: Stuart Armstrong

LAB HEAD: Dr Dong Xia

PROVIDER: PXD019354 | Pride | 2021-09-09

REPOSITORIES: Pride

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Publications

Proteomic Characterization of Host-Pathogen Interactions during Bovine Trophoblast Cell Line Infection by <i>Neospora caninum</i>.

Regidor-Cerrillo Javier J Xia Dong D Jiménez-Pelayo Laura L García-Sánchez Marta M Collantes-Fernández Esther E Randle Nadine N Wastling Jonathan J Ortega-Mora Luis-Miguel LM Horcajo Pilar P

Pathogens (Basel, Switzerland) 20200915 9

Despite the importance of bovine neosporosis, relevant knowledge gaps remain concerning the pathogenic mechanisms of <i>Neospora caninum</i>. Infection of the placenta is a crucial event in the pathogenesis of the disease; however, very little is known about the relation of the parasite with this target organ. Recent studies have shown that isolates with important variations in virulence also show different interactions with the bovine trophoblast cell line F3 in terms of proliferative capacity ...[more]

PMID: 32942559

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Project description:The experiment investigates bovine gene expression in response to BW720c treatment in uninfected and Theileria annulata-infected cell cultures Theileria annulata, an intracellular parasite of bovine lymphoid cells, induces substantial phenotypic alterations to its host cell including continuous proliferation, cytoskeletal changes and resistance to apoptosis. While parasite induced modulation of host cell signal transduction pathways and NFκB activation are established, there remains considerable speculation on the complexities of the parasite directed control mechanisms that govern these radical changes to the host cell. Our objectives in this study were to provide a comprehensive analysis of the global changes to host cell gene expression with emphasis on those that result from direct intervention by the parasite. By using comparative microarray analysis of an uninfected bovine cell line and its Theileria infected counterpart, in conjunction with use of the specific parasitacidal agent, buparvaquone, we have identified a large number of host cell gene expression changes that result from parasite infection. Our results indicate that the viable parasite can irreversibly modify the transformed phenotype of a bovine cell line. 50% of genes with altered expression failed to show a reversible response to parasite death, a possible contributing factor to initiation of host cell apoptosis. The genes that did show an early predicted response to loss of parasite viability highlighted a sub-group of genes that are likely to be under direct control by parasite infection. Network and pathway analysis demonstrated that this sub-group is significantly enriched for genes involved in regulation of expression and chromatin modification. The results provide evidence that the Theileria parasite has the regulatory capacity to generate widespread change to host cell gene expression in a complex and reversible manner. Six experimental conditions with three replicates per condition. Total RNA prepared from cell cultures. BL20 (uninfected bovine lymphosarcoma cell line), BL20 24 hours post-BW720c treatment, BL20 48 hours post-BW720c, TBL (T. annulata infected bovine cell line), TBL 24 hours post-BW720c, TBL 48 hours post-BW720c. Each hydridisation represents bovine and parasite gene expression on a single channel and 2 technical replicates of each probeset are represented on the chip.

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Dataset Information

Proteomic characterisation of host-pathogen interactions during bovine trophoblast cell line infection by Neospora caninum

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Publications

Proteomic Characterization of Host-Pathogen Interactions during Bovine Trophoblast Cell Line Infection by <i>Neospora caninum</i>.

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