Dataset Information

A novel mobile genomic island: introducing ‘cephalosporinase morons’ in Enterobacteriaceae

ABSTRACT: The exchange of mobile genomic islands (MGIs) between microorganisms is often mediated by phages. As a consequence, not only phage genes are transferred, but also genes that have no particular function in the phage's lysogenic cycle. If they provide benefits to the phage's host, such genes are referred to as ‘morons’. The present study was aimed at characterizing a set of Enterobacter cloacae, Klebsiella pneumoniae and Escherichia coli isolates with exceptional antibiotic resistance phenotypes from patients in a neonatal ward. Unexpectedly, these analyses unveiled the existence of a novel family of closely related MGIs in Enterobacteriaceae. The respective MGI from E. cloacae was named MIR17-GI. Importantly, our observations show that MIR17-GI-like MGIs harbor genes associated with high-level resistance to cephalosporins. Further, we show that MIR17-GI-like islands are associated with integrated P4-like prophages. This implicates phages in the spread of cephalosporin resistance amongst Enterobacteriaceae. The discovery of a novel family of MGIs spreading ‘cephalosporinase morons’ is of high clinical relevance, because high-level cephalosporin resistance has serious implications for the treatment of patients with Enterobacteriaceal infections.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Enterobacter Cloacae

SUBMITTER: Andreas Otto

LAB HEAD: Andreas Otto

PROVIDER: PXD007113 | Pride | 2018-08-21

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	13047T_cyt_exp_vs_stat.sf3	Other
	141024K_cyt_exp_vs_stat.sf3	Other
	14260199101_cyt_exp_vs_stat.sf3	Other
	14280161202_cyt_exp_vs_stat.sf3	Other
	14290088701_cyt_exp_vs_stat.sf3	Other

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Publications

An ancient family of mobile genomic islands introducing cephalosporinase and carbapenemase genes in Enterobacteriaceae.

Nepal Suruchi S Bonn Florian F Grasso Stefano S Stobernack Tim T de Jong Anne A Zhou Kai K Wedema Ronald R Rosema Sigrid S Becher Dörte D Otto Andreas A Rossen John W JW van Dijl Jan Maarten JM Bathoorn Erik E

Virulence 20180101 1

The exchange of mobile genomic islands (MGIs) between microorganisms is often mediated by phages, which may provide benefits to the phage's host. The present study started with the identification of Enterobacter cloacae, Klebsiella pneumoniae and Escherichia coli isolates with exceptional cephalosporin and carbapenem resistance phenotypes from patients in a neonatal ward. To identify possible molecular connections between these isolates and their β-lactam resistance phenotypes, the respective ba ...[more]

PMID: 30101693

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