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Analysis of Bacillus subtilis intracellular proteome under exposition to silver nanoparticles

ABSTRACT: Nanoparticles are more and more used in industrial process, food, medicinal or daily goods. At the end of their life, most of them are discharged in the environment where they impact the ecological equilibrium. Bacteria are one of the first targets of the nanoparticles in the environment. To evaluate the impact of silver nanoparticles on the physiology of Bacillus subtilis, we studied the intracellular proteome of bacteria exposed to different nanoparticles during the stationary phase.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Bacillus Subtilis Subsp. Subtilis Str. Ncib 3610

SUBMITTER: Yohann Couté

LAB HEAD: Yohann Coute

PROVIDER: PXD007128 | Pride | 2018-05-14

REPOSITORIES: pride

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Publications

The poly-gamma-glutamate of Bacillus subtilis interacts specifically with silver nanoparticles.

Eymard-Vernain Elise E Coute Yohann Y Adrait Annie A Rabilloud Thierry T Sarret Géraldine G Lelong Cécile C

PloS one 20180529 5

For many years, silver nanoparticles, as with other antibacterial nanoparticles, have been extensively used in manufactured products. However, their fate in the environment is unclear and raises questions. We studied the fate of silver nanoparticles in the presence of bacteria under growth conditions that are similar to those found naturally in the environment (that is, bacteria in a stationary phase with low nutrient concentrations). We demonstrated that the viability and the metabolism of a gr ...[more]

PMID: 29813090

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