Proteomics

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EASI-tag enables accurate multiplexed and interference-free MS2-based proteome quantification

ABSTRACT: We developed EASI-tag (Easily Abstractable Sulfoxide-based Isobaric tag), a new generation of amine-derivatizing and sulfoxide-containing isobaric labelling reagents, which dissociate at low collision energy and generate peptide-coupled, interference-free reporter ions with high yield. Efficient isolation of 12C precursors and quantification at the MS2 level enable accurate determination of quantitative differences between multiplexed samples. EASI-tag makes isobaric labeling applicable to any bottom up proteomics application and to benchtop mass spectrometers.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human) Saccharomyces Cerevisiae (baker's Yeast)

TISSUE(S): Epithelial Cell, Hela Cell

SUBMITTER: Sebastian Virreira Winter  

LAB HEAD: Matthias Mann

PROVIDER: PXD007165 | Pride | 2018-06-21

REPOSITORIES: Pride

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20170510_QEp1_SeVW_SA_HeLa_TMTzero_NCEramp_10-34_fraction_1.raw Raw
20170510_QEp1_SeVW_SA_HeLa_TMTzero_NCEramp_10-34_fraction_2.raw Raw
20170510_QEp1_SeVW_SA_HeLa_TMTzero_NCEramp_10-34_fraction_3.raw Raw
20170510_QEp1_SeVW_SA_HeLa_TMTzero_NCEramp_10-34_fraction_4.raw Raw
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EASI-tag enables accurate multiplexed and interference-free MS2-based proteome quantification.

Virreira Winter Sebastian S   Meier Florian F   Wichmann Christoph C   Cox Juergen J   Mann Matthias M   Meissner Felix F  

Nature methods 20180618 7

We developed EASI-tag (easily abstractable sulfoxide-based isobaric-tag), a new type of amine-derivatizing and sulfoxide-containing isobaric labeling reagents for highly accurate quantitative proteomics analysis using mass spectrometry. We observed that EASI-tag labels dissociate at low collision energy and generate peptide-coupled, interference-free reporter ions with high yield. Efficient isolation of <sup>12</sup>C precursors and quantification at the MS2 level allowed accurate determination  ...[more]

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