Dataset Information

Comprehensive profiling of lysine ubiquitome reveals diverse functions of lysine ubiquitination in common wheat

ABSTRACT: Protein ubiquitination, which is a major post-translational modifications that occurs in eukaryotic cells, is involved in diverse biological processes. To date, large-scale profiling of the ubiquitome in common wheat has not been reported, despite its status as the major cereal crop in the world. Here, we performed the first ubiquitome analysis of the common wheat (Triticum aestivum L.) variety, Aikang 58. Overall, 433 lysine modification sites were identified in 285 proteins in wheat seedlings, and four putative ubiquitination motifs were revealed. Moreover, ubiquitylated lysines were found to have a significantly different preference for secondary structures when compared with the all lysines. Bioinformatics analysis revealed that the ubiquitinated proteins were involved in a wide variety of biological processes and diverse subcellular localizations. In accordance with previous studies, proteins related to binding and catalytic activity were predicted to be the preferential targets of lysine ubiquitination. Besides, protein interaction network analysis reveals that diverse interactions are modulated by protein ubiquitination. The results presented herein provide a global view of the ubiquitome in common wheat for the first time and lays a foundation for exploring the physiological role of lysine ubiquitination in wheat and other plants.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Triticum Aestivum (wheat)

TISSUE(S): Leaf

SUBMITTER: Ning Zhang

LAB HEAD: Feng Chen

PROVIDER: PXD007243 | Pride | 2017-11-01

REPOSITORIES: Pride

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Publications

Comprehensive profiling of lysine ubiquitome reveals diverse functions of lysine ubiquitination in common wheat.

Zhang Ning N Zhang Lingran L Shi Chaonan C Tian Qiuzhen Q Lv Guoguo G Wang Ying Y Cui Dangqun D Chen Feng F

Scientific reports 20171019 1

Protein ubiquitination, which is a major post-translational modifications that occurs in eukaryotic cells, is involved in diverse biological processes. To date, large-scale profiling of the ubiquitome in common wheat has not been reported, despite its status as the major cereal crop in the world. Here, we performed the first ubiquitome analysis of the common wheat (Triticum aestivum L.) variety, Aikang 58. Overall, 433 lysine modification sites were identified in 285 proteins in wheat seedlings, ...[more]

PMID: 29051560

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