Dataset Information

Mechanism of persistence of the ammonia oxidising bacteria - Nitrosomonas to the the biocide Free Nitrous Acid (FNA)

ABSTRACT: This study shows the molecular mechanism by which the ammonia oxidising bacteria- Nitrosomonas is able to persist in high concentrations of FNA.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Uncultured Nitrosomonas Sp. B7

SUBMITTER: Andrew Laloo

LAB HEAD: Dr Phil Bond

PROVIDER: PXD007514 | Pride | 2023-06-02

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	11082016Andrew_Final.group	Other
	20160323_Andrew_AWMC_IDA_0-1.MGF	Mgf
	20160323_Andrew_AWMC_IDA_0-1.wiff	Wiff
	20160323_Andrew_AWMC_IDA_0-1.wiff.scan	Wiff
	20160323_Andrew_AWMC_IDA_0-3.MGF	Mgf

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Publications

Mechanisms of Persistence of the Ammonia-Oxidizing Bacteria Nitrosomonas to the Biocide Free Nitrous Acid.

Laloo Andrew E AE Wei Justin J Wang Dongbo D Narayanasamy Shaman S Vanwonterghem Inka I Waite David D Steen Jason J Kaysen Anne A Heintz-Buschart Anna A Wang Qilin Q Schulz Benjamin B Nouwens Amanda A Wilmes Paul P Hugenholtz Philip P Yuan Zhiguo Z Bond Philip L PL

Environmental science & technology 20180416 9

Free nitrous acid (FNA) exerts a broad range of antimicrobial effects on bacteria, although susceptibility varies considerably among microorganisms. Among nitrifiers found in activated sludge of wastewater treatment processes (WWTPs), nitrite-oxidizing bacteria (NOB) are more susceptible to FNA compared to ammonia-oxidizing bacteria (AOB). This selective inhibition of NOB over AOB in WWTPs bypasses nitrate production and improves the efficiency and costs of the nitrogen removal process in both t ...[more]

PMID: 29620869

Similar Datasets

Project description:The abundance of bacterial (AOB) and archaeal (AOA) ammonia oxidisers, assessed using quantitative PCR measurements of their respective a-subunit of the ammonia monooxygenase (amoA) genes, and ammonia oxidation rates were measured in four contrasting coastal sediments in the Western English Channel. Sediment was sampled bimonthly from July 2008 to May 2011, and measurements of ammonia oxidiser abundance and activity compared to a range of environmental variables including salinity, temperature, water column nutrients and sediment carbon and nitrogen content. Despite a higher abundance of AOA amoA genes within all sediments, and at all time-points, rates of ammonia oxidation correlated with AOB and not AOA amoA gene abundance. Other than ammonia oxidation rate, sediment particle size was the only variable that correlated with the spatial and temporal patterns of AOB amoA gene abundance, implying a preference of the AOB for larger sediment particles. This is possibly due to deeper oxygen penetration into the sandier sediments, increasing the area available for ammonia oxidation to occur, higher concentrations of inhibitory sulphide with pore waters of muddier sediments or a combination of both oxygen and sulphide concentrations. Similar to many other temporal studies of nitrification within estuarine and coastal sediments, decreases in AOB amoA gene abundance were evident during summer and autumn, with maximum abundance and ammonia oxidation rates occurring in winter and early spring. The lack of correlation between AOA amoA gene abundance and ammonium oxidation rate suggests an alternative role for amoAÂ-carrying AOA within these sediments. Two color array (Cy3 and Cy5): the universal standard 20-mer oligo is printed to the slide with a 70-mer oligo (an archetype). Environmental DNA sequences (fluoresced with Cy3) within 15% of the 70-mer conjugated to a 20-mer oligo (fluoresced with Cy5) complementary to the universal standard will bind to the oligo probes on the array. Signal is the ratio of Cy3 to Cy5. Three replicate probes were printed for each archetype. Two replicate arrays were run on duplicate targets.