Dataset Information

Protein variability in cerebrospinal fluid and its possible implications for neurological protein biomarker research

ABSTRACT: Investigation of inter- and intraindividual variability of the cerebrospinal fluid proteome globally and with a specific focus on disease biomarkers described in literature of neurologically unimpaired healthy controls. For further studies we calculated for all proteins a reference change value (RCV).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cerebrospinal Fluid

SUBMITTER: Steffen Kösters

LAB HEAD: Katrin Marcus

PROVIDER: PXD007555 | Pride | 2019-11-12

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 38

Publications

Protein variability in cerebrospinal fluid and its possible implications for neurological protein biomarker research.

Schilde Lukas M LM Kösters Steffen S Steinbach Simone S Schork Karin K Eisenacher Martin M Galozzi Sara S Turewicz Michael M Barkovits Katalin K Mollenhauer Brit B Marcus Katrin K May Caroline C

PloS one 20181129 11

Cerebrospinal fluid is investigated in biomarker studies for various neurological disorders of the central nervous system due to its proximity to the brain. Currently, only a limited number of biomarkers have been validated in independent studies. The high variability in the protein composition and protein abundance of cerebrospinal fluid between as well as within individuals might be an important reason for this phenomenon. To evaluate this possibility, we investigated the inter- and intraindiv ...[more]

PMID: 30496192

Similar Datasets

Project description:Acute inflammatory demyelinating polyneuropathy (AIDP) - one of the forms of Guillain-Barre syndrome (GBS) - is a rare and severe disorder of the peripheral nervous system that also has an unknown etiology. One of the hallmarks of AIDP pathogenesis is a significantly elevated cerebrospinal fluid (CSF) protein level in comparison with the peptidome/proteome profile of a representative number of CSF samples obtained from AIDP and multiple sclerosis (MS) patients and control patients. In total, 2355 peptide fragments related to 795 proteins were found in CSF samples from AIDP patients, 1066 peptide fragments of 253 proteins in samples from MS patients, and 774 peptide fragments of 220 proteins from control patients. Proteomic studies detected 1103 proteins in the control cohort and 1402 proteins in the AIDP cohort. Our dataset suggests that the proteome of the CSF from AIDP and control patients overlaps by as much as 67%, whereas the peptidome is only 21.5% identical. Comparison of the peptidomic and proteomic data revealed that 80% of the peptides in the CSF of AIDP patients correspond to proteins that are not present in proteomic dataset. Importantly, a considerable number of differentially represented peptides are related to the proteins involved in the arrangement of the axonal domains. The observed peptidomic dataset indicates that specific proteins, which participate in the arrangement of the myelin sheath in the nodes of Ranvier, are degraded in the course of AIDP. Here, we show that the proteins that are overrepresented in the CSF of AIDP patients are partially linked with defensive responses to bacteria and viruses. These observations are in line with the upregulation of CSF cytokines associated with innate immunity, which in turn surprisingly suggests that the destruction of axons is not accompanied by any indications of a distinct adaptive autoimmune process. We believe that the presented proteomic and peptidomic dataset of the CSF from AIDP patients will provide useful information regarding the mechanisms of AIDP pathogenesis in comparison with MS, which is the prolonged but incurable autoimmune degradation of the central nervous system.

			Action	DRS
	K1.1QEXI16153.raw	Raw
	K1.2QEXI16195.raw	Raw
	K1.3QEXI16203.raw	Raw
	K13.1QEXI16187.raw	Raw
	K13.2QEXI16201.raw	Raw