Proteomics

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Protein profile at different cellular fractions of Mycobacterium tuberculosis strains after exposure to Isoniazid


ABSTRACT: Out of the 10 million of tuberculosis (TB) cases estimated in the world, around 14% are isoniazid (INH) resistant among new cases and 29% among previously treated cases in the last decade. INH is one of the oldest but also one of the more potent drugs to eliminate Mycobacterium tuberculosis (Mtb), the causing agent of TB. Because of the efficiency of isoniazid (INH) against Mycobacterium tuberculosis (Mtb), many studies are still focused in better understand its role in different bacterial metabolic pathways. We recently conducted a study that evaluated the changes in the protein abundance at different cellular fractions when clonal strains of Mtb developed INH resistance in the clinical and laboratory setting. Here, we want to establish which of the protein changes occurred or started because of the initial exposure to INH. Additionally, we wanted to evaluate if those changes happen differently in strains that are sensitive or resistant to INH, evaluating different cellular compartments from two different genetic lineages of Mtb. For this purpose, we analyzed the proteome of each cellular compartment (cytosol, cell wall, membrane and secreted proteins) through liquid chromatography (nano-HPLC) coupled to mass spectrometry using the Orbitrap Velos instrument and a t-test to perform the statistical analysis for each pair comparison.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mycobacterium Tuberculosis H37rv

TISSUE(S): Prokaryotic Cell

DISEASE(S): Pulmonary Tuberculosis

SUBMITTER: Luisa Nieto Ramirez  

LAB HEAD: Karen M. Dobos, Ph.D.

PROVIDER: PXD007588 | Pride | 2019-06-17

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
CFP_21-1.mzXML Mzxml
CFP_21-1.mzid.gz Mzid
CFP_21-1.mzid_CFP_21-1.MGF Mzid
CFP_21-1.mzid_CFP_21-1.pride.mgf.gz Mzid
CFP_21-1.pride.mztab.gz Mztab
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Publications

Protein profile of different cellular fractions from <i>Mycobacterium tuberculosis</i> strains after exposure to isoniazid.

Nieto Ramirez Luisa María LM   Mehaffy Carolina C   Dobos Karen M KM  

Data in brief 20190425


Different biochemical studies looking for the effect of INH on the physiology of <i>Mycobacterium tuberculosis (Mtb)</i> have been conducted. Here, we present a detailed analysis, looking at the protein variation in the <i>Mtb</i> cell due to exposure of sub-inhibitory concentrations of INH, evaluating three different variables: cellular fraction, genetic lineage, and INH phenotypic profile. Mass spectrometry analysis demonstrated that the most significantly affected cellular fraction was the me  ...[more]

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